Reducing antimicrobial resistant bacteria with the use of cold atmospheric plasma: Mechanism of action and influence on mutation and horizontal gene t

使用冷大气等离子体减少抗菌药物耐药性细菌：作用机制以及对突变和水平基因t的影响

• 批准号: 2888329
• 金额: --
• 依托单位: University of Bristol
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2888329
• 关键词: Reducing; antimicrobial; resistant; bacteria; use

Project description:The widespread use and misuse of antibiotics in animal production has led to the emergence of antimicrobial-resistantAMR bacteria on farms, which adds to the global burden of AMR, which has significant impacts on human health andfood security. Significant efforts to limit AMR on animal farms have so far focused on controlling the supply and use ofantimicrobial drugs. However, evidence shows that AMR pathogenic bacteria can persist and spread despite reducingantimicrobial use to almost zero. AMR development and persistence in livestock is therefore not only an issue to beaddressed by reducing antimicrobial use. We have shown that cleaning and disinfection have a very important role toplay, but the extensive use of chemical disinfectants has been associated with the emergence of AMR and has aconsiderable environmental impact. Therefore, new sustainable strategies for decontamination at the farm level arenecessary. Cold atmospheric plasma (CAP) is a novel, residue and antibiotic-free, non-thermal technology shown to haveantibacterial properties in a variety of different settings. Plasma describes the state of an ionised gas (4th state ofmatter), with natural examples including the northern lights, lightening and solar winds. In our lab, we produce CAP byexcitation of gas molecules using electrical discharges. CAP has the potential to inhibit bacteria due to the reactivemolecules and species it contains (e.g. oxygen/nitrogen reactive species). Our previous studies have proved that CAP iseffective in eliminating bacterial pathogens on different surfaces. This project aims to explore the viability of using CAPto reduce AMR bacterial contamination on surfaces frequently found on farms, and the biological effects of CAP onbacteria. You will investigate: i) the efficacy of CAP against AMR bacteria previously identified on farms; ii) the CAP-mediated mechanism of action against AMR bacteria using functional genomics; iii) The influence of CAP on mutationand horizontal transfer of AMR in bacteria; iv) the efficacy of CAP in controlling AMR bacteria on abiotic surfaces. The successful student will join a consortium of two interdisciplinary research groups with access to world-class researchfacilities at University of Bristol (molecular bacteriology, proteomics/functional genomics) and University of the West ofEngland-Bristol (cold plasma technology, microbiology). The student will receive excellent training and meaningfulsupport from their supervisory team, and develop the technical skills and enterprising mind-set that employers seek.

项目简介：动物生产中抗生素的广泛使用和滥用，导致农场出现耐药细菌，加重了全球AMR负担，对人类健康和粮食安全产生重大影响。迄今为止，限制动物养殖场抗菌素耐药性的重大努力主要集中在控制抗菌药物的供应和使用上。然而，有证据表明，尽管抗菌药物的使用量几乎为零，但抗菌素耐药性致病菌仍能持续存在并传播。因此，牲畜中抗菌素耐药性的发展和持久性不仅是一个需要通过减少抗菌药物使用来解决的问题。我们已经表明，清洁和消毒发挥着非常重要的作用，但化学消毒剂的广泛使用与抗菌素耐药性的出现有关，并对环境产生了相当大的影响。因此，有必要在农场层面采取新的可持续净化战略。冷大气等离子体 (CAP) 是一种新型、无残留、无抗生素的非热技术，在各种不同的环境下均具有抗菌特性。等离子体描述了电离气体的状态（物质的第四态），自然的例子包括北极光、闪电和太阳风。在我们的实验室中，我们通过放电激发气体分子来生产 CAP。由于其含有反应性分子和物质（例如氧/氮反应性物质），CAP 具有抑制细菌的潜力。我们前期的研究已经证明CAP对于消除不同表面的细菌病原体是有效的。该项目旨在探索使用 CAP 减少农场常见表面上的 AMR 细菌污染的可行性，以及 CAP 对细菌的生物效应。您将研究： i) CAP 对先前在农场发现的 AMR 细菌的功效； ii) 使用功能基因组学研究 CAP 介导的针对 AMR 细菌的作用机制； iii) CAP对细菌AMR突变和水平转移的影响； iv) CAP 在控制非生物表面 AMR 细菌方面的功效。成功的学生将加入由两个跨学科研究小组组成的联盟，并可使用布里斯托大学（分子细菌学、蛋白质组学/功能基因组学）和西英格兰大学布里斯托尔大学（冷等离子体技术、微生物学）的世界一流研究设施。学生将获得导师团队的出色培训和有意义的支持，并培养雇主所寻求的技术技能和进取心态。