Functional role of microRNA in acute myeloid leukemia stem cells and their normal

microRNA在急性髓系白血病干细胞及其正常状态中的功能作用

• 批准号: 7302634
• 负责人: CHRISTOPHER Y PARK
• 金额: $ 13.75万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-03 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7302634
• 关键词: Accounting; Acute Myelocytic Leukemia; Adult; Anatomy; Antibody Therapy; Apoptosis; Biological Assay; Blast Cell; Bone Marrow Transplantation; Cancer Biology; Cell physiology; Cell surface; Cells; Cellular biology; Chromosomal translocation; Chronic Myeloid Leukemia; Chronic Phase; Clinical; Commit; Communities; Comprehensive Cancer Center; Crowding; Culture Techniques; Data; Daughter; Depth; Development; Development Plans; Developmental Process; Diagnosis; Diagnostic; Disease; Engraftment; Environment; Epigenetic Process; Event; Exhibits; Experimental Pathology; Fellowship; Figs - dietary; Future; Gene Fusion; Gene Mutation; Gene Targeting; Genes; Genetic; Genome Stability; Goals; Hematopathology; Hematopoiesis; Hematopoietic; Hematopoietic stem cells; Housing; Human; Human Development; Immune; Immunology; Immunophenotyping; In Vitro; Institutes; Investigation; Laboratories; Lead; Lesion; Libraries; Malignant Neoplasms; Malignant lymphoid neoplasm; Methylcellulose; MicroRNAs; Modeling; Molecular; Molecular Profiling; Molecular Target; Mus; Myeloid Leukemia; Oncogenes; Pathogenesis; Pathology; Pathway interactions; Patients; Pediatric Hematology/Oncology; Pharmaceutical Preparations; Phenotype; Placement; Play; Polymerase Chain Reaction; Population; Principal Investigator; Proliferating; Property; Protein Overexpression; Proteins; Proto-Oncogenes; Regenerative Medicine; Research; Research Personnel; Residencies; Resources; Retroviridae; Role; Sampling; Staging; Standards of Weights and Measures; Stem cells; System; Techniques; Testing; Therapeutic; Thinking; Time; Tissues; Tort; Training; Transplantation; Triage; Tumor Suppressor Genes; Tumor Suppressor Proteins; Universities; Work; Xenograft Model; Xenograft procedure; base; c-myc Genes; cancer stem cell; carcinogenesis; career; daughter cell; design; granulocyte; improved; in vitro Assay; in vivo; innovation; insight; knock-down; leukemia; leukemic stem cell; leukemogenesis; macrophage; member; mouse model; novel; progenitor; prognostic; programs; research study; self-renewal; skills; stem; tool; tumor

DESCRIPTION (provided by applicant): This 5 year-proposal describes a comprehensive plan for the development of a career in experimental pathology. The principal investigator completed anatomic pathology residency and hematopathology fellowship training at Stanford University and has spent the past two years honing his skills as a diagnostic hematopathologist while spending the majority of his time developing the technical and intellectual skills that will allow him to become an independent investigator. The proposed work will take place in the laboratory of Irving L. Weissman, an internationally recognized leader in the field of stem cell biology, cancer stem cell biology, and hematopoiesis. Given the breadth and depth of intellectual and technological resources, Dr. Weissman's lab represents a unique environment, geared not only to allowing highly creative and innovative projects to come to fruition, but also to train future independent investigators. Given the growing evidence that microRNAs play a role in the development of human cancers, this proposal represents a timely investigation into the pathogenesis of human acute myeloid leukemia (AML). Unlike conventional cancer biology research, the proposed studies will focus on the tumor-initiating, or stem cells, of AML. Because of the unique properties of leukemia stem cells (LSC), including the capacity to initiate tumors, self-renew, and recapitulate disease in xenograft models, it is imperative that we understand the molecular basis of the AML LSC biologic properties. Because of the unique experimental challenges associated with working with primary human samples, a great deal of time has been, and will continue to be, invested in developing improved models to study the role of microRNAs in human AML. The Stanford Institute for Stem Cell Biology and Regenerative Medicine is an ideal setting for these studies. In addition to housing a community of cancer and stem cell biologists, there are numerous opportunities to collaborate with members of other departments through interdisciplinary programs including the Stanford Comprehensive Cancer Center, Immunology Program, and many others. Given the strength of Stanford's clinical divisions (adult and pediatric hematology/oncology, bone marrow transplant, hematopathology), there is significant tissue access as well as opportunities to work with clinical colleagues.

描述（由申请人提供）：这份5年计划书描述了实验病理学职业发展的全面计划。首席研究员在斯坦福大学完成了解剖病理学住院医师和血液病理学奖学金培训，在过去的两年里，他花了大部分时间来磨练他作为诊断血液病理学家的技能，同时花了大部分时间来发展技术和智力技能，这将使他成为一名独立的研究者。