Electrophysiology/Cell Culture Core

电生理学/细胞培养核心

• 批准号: 7030418
• 负责人: Gail A Hecht
• 金额: $ 13.37万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-21 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7030418
• 关键词: biomedical facility; cell line; electrophysiology; fluorimetry; gastrointestinal epithelium; tissue /cell culture

Electrophysiology/Cell Culture Core is designed to provide PPG investigators with an array of commonly utilized cultured human intestinal epithelial cell lines (Caco-2, T84, HT-29, and NCM460) and access to a multitude of electrophysiological measurements on both cultured epithelial sheets and native tissues. Both Core components house state-of-the-art equipment, which is run by personnel with full technical expertise so that training, assistance with experimental design, data collection, data analysis and interpretation are provided. This Core is under dual direction by Drs. Gail Hecht and Mrinalini Rao. They both have extensive experience in all aspects of electrophysiological measurements in cultured intestinal epithelia, native intestinal tissue, and primary intestinal cells. The Specific Aims are to provide the investigators and projects within this Program Project with human cultured intestinal epithelial cells including Caco-2, T84, HT-29, and NCM460 grown on plastic or permeable (Transwell) filters; electrophysiological measurements in Ussing chambers including short circuit current (Isc), transepithelial electrical resistance (TER), ion flux analysis, on both cultured intestinal sheets and native intestinal tissues; and spectrofluorometric measurements as a complementary approach to assessing ion transport. The provision of these cells for electrophysiologic measurements will facilitate and expedite the proposed experiments of all of the Program investigators and will standardize the conditions under which these cells are grown thus allowing for direct correlation of data obtained for each of the Projects.

电生理学/细胞培养核心旨在为 PPG 研究人员提供一系列常见的 利用培养的人肠上皮细胞系（Caco-2、T84、HT-29 和 NCM460）并获得 对培养的上皮片和天然组织进行大量的电生理学测量。两个都 核心部件拥有最先进的设备，由具有全面技术专业知识的人员操作，因此 培训、实验设计协助、数据收集、数据分析和解释是 假如。该核心由博士双重指导。盖尔·赫克特和姆里纳利尼·拉奥。他们俩都有广泛的 在培养的肠上皮、天然肠道的电生理测量的各个方面都有经验 组织和原代肠细胞。具体目标是为该领域内的研究人员和项目提供帮助 人类培养肠上皮细胞项目，包括 Caco-2、T84、HT-29 和 NCM460 在塑料或渗透性（Transwell）过滤器上生长；使用室中的电生理测量 包括短路电流 (Isc)、跨上皮电阻 (TER)、离子通量分析 培养的肠片和天然肠组织；和荧光分光光度测量 评估离子传输的补充方法。提供这些细胞用于电生理学 测量将促进和加速所有计划研究人员的拟议实验 将标准化这些细胞的生长条件，从而实现数据的直接关联 为每个项目获得。