Ameloblastin Function in the Periodontal Ligament

描述（由申请人提供）：深度牙周治疗的最严重并发症之一是称为牙周强直的过程：牙槽骨和牙根之间形成坚固的骨性附着。因此，我们提出了这样一个问题，即牙槽骨和牙根牙骨质之间的非矿化牙周膜间隙是如何维持的。为了确定可能有助于HERS促进的非矿化牙周膜间隙的建立和维持的候选分子，我们在具有同时牙周组织再生和牙周膜维持的牙齿移动模型中进行了基因阵列分析（Holliday等人，2005）。从这些屏幕上，成釉蛋白出现作为一个HRES相关的基因产物，在牙周再生和韧带重建过程中显着上调。在此基础上，我们进行了一些初步的研究，以测试成釉蛋白对牙周膜细胞矿化行为的影响。这些研究结果表明，成釉蛋白显着抑制牙周膜细胞的矿化。在这里，我们提出了一系列的研究，以进一步调查成釉蛋白对牙周膜细胞的矿化行为的影响，并确定成釉蛋白可能会影响牙周膜在我们的牙周再生模型。 叙述：牙龈疾病最严重的并发症之一是称为强直的过程：颌骨和牙根之间形成坚固的骨性附着。在这里，我们将进行一系列的研究，以调查一种独特的基因产物（称为成釉蛋白），以防止强直的效果。这些研究可能最终导致牙龈疾病的新疗法。

DESCRIPTION (provided by applicant): One of the most severe complications of deep-scaling periodontal therapy is a process called periodontal ankylosis: the formation of a solid bony attachment between alveolar bone and tooth roots. We have therefore asked the question as to how this non-mineralized periodontal ligament space between alveolar bone and root cementum is maintained. In order to determine candidate molecules that might contribute to the establishment and maintenance of a non- mineralized periodontal ligament space facilitated by HERS, we have performed gene array analyses in a tooth movement model featuring simultaneous periodontal tissue regeneration and periodontal ligament maintenance (Holliday et al. 2005). From these screens, ameloblastin emerged as a HERS-related gene product that was significantly upregulated during periodontal regeneration and ligament remodeling. Following this lead, we have performed a number of preliminary studies to test the effect of ameloblastin on the mineralization behavior of periodontal ligament cells. Results from these studies suggested that ameloblastin significantly inhibits mineralization of periodontal ligament cells. Here we propose a series of studies to further investigate the effect of ameloblastin on the mineralization behavior of periodontal ligament cells and to determine how ameloblastin might affect the periodontal ligament in our periodontal regeneration model. Narrative: One of the most severe complications of gum diseases is a process called ankylosis: the formation of a solid bony attachment between jaw bone and tooth roots. Here we will conduct a series of studies to investigate the effect of a unique gene product (called ameloblastin) to prevent ankylosis. These studies might eventually lead to novel cures for gum diseases.