Continuous Chromosome Sorting with Micro/nanofluidics

描述(由申请人提供)： 对长的基因组DNA的单分子分析将提供更多关于基因组结构异常/变异的知识，并改善对它们与癌症关系的理解。该项目的长期目标是开发一种完全集成的芯片和读取器，能够对大型自然状态基因组材料进行单分子分析。预期的实施例将允许直接可视化和分析直接从样本(可能是单个细胞)中提取的DNA的染色体和百万碱基片段，分辨率为千碱基以下。此外，该芯片将以高通量方式容纳对单个DNA分子的大规模并行分析，从而及时提供统计上的相关数据。 由于大多数疾病相关的基因座都位于特定的染色体上，因此在纳米流体中进行进一步的单分子水平分析之前能够对其进行预分类是非常有价值的。为了帮助实现这一目标，我们建议研究将普林斯顿大学斯特姆教授开发的微流控粒子分类技术与BioNanomatrix的纳米流控DNA分析技术相结合的可能性。一个基于大规模并行连续样品分析的微/纳米流体标准化平台可以显著降低成本，并为未来患者护理中一致的高通量基因组分析奠定基础。

DESCRIPTION (provided by applicant): Single molecule analysis of long, genomic DNA will provide greater knowledge of genomic structural aberrations/variations and improved understanding of their association with cancer. The long-term goal of this project is to develop a fully integrated chip and reader capable of single molecule analysis of large native state genomic material. The anticipated embodiment will permit direct visualization and analysis of chromosomal and megabase fragments of DNA extracted directly from a sample (possibly a single cell) with sub-kilobase resolution. Furthermore, the chip will accommodate massively parallel analyses of individual DNA molecules in a high- throughput manner thus providing statistically relevant data in a timely fashion. As most disease related loci are located on specific chromosomes, it is of great value to be able to pre-sort them prior to further single molecule level analysis in nanofluidics. In order to help reach this objective, we propose investigating the possibility of integrating microfluidic particle sorting technology developed under Prof Sturm at Princeton University with BioNanomatrix's nanofluidic DNA analysis technology. A micro/nanofluidic standardized platform based on continuous sample analysis in massive parallel fashion could dramatically reduce the cost and serve as a basis for consistent, high-throughput genomic analyses in future patient care.