Mosaic Mouse Models of Human Cancer

Core C通过以下方式为小鼠马赛克模型的生成和表征提供了基本支持 项目调查员。Core支持的模型包括myc诱导的淋巴瘤模型、 肝细胞癌的马赛克模型和乳腺癌的马赛克模型--这三种模型 ARE由计划调查人员开发，并在整个计划中使用。核心将保持 提供祖细胞或受体动物来源以供生产的一系列动物群体 老鼠马赛克。它还将使用协议分离造血祖细胞、肝脏祖细胞和乳腺祖细胞 由方案建立，供个别项目使用，并培训和协助项目调查人员 在小鼠嵌合体的产生和肿瘤发生的监测方面。最后，核心包含了高度的 熟练的病理学家和组织学服务，能够对各种病理组织进行详细的定性 表型。这些服务为计划带来了独特、高质量和经济高效的服务 使每个项目能够有效地使用活体模型来探索正常和肿瘤生物学的各个方面。

Core C provides essential support for the generation and characterization of mouse mosaic models by Program investigators. The models supported by the Core include a myc-induced lymphoma model, a mosaic model of hepatocellular carcinoma, and a mosaic model of mammary carcinoma - all three of which are were developed by Program investigators and are used throughout the Program. The Core will maintain a series of animal colonies that provide a source of progenitor cells or recipient animals for the production of mouse mosaics. It will also isolate hematopoietic, liver, and mammary progenitor cells using protocols established by the Program for use by individual Projects, and train and assist Project investigators in the generation of mouse mosaics and in monitoring of tumorigenesis. Finally, the Core incorporates a highly skilled pathologist and histology service to enable a detailed characterization of various pathological phenotypes. These services bring to the Program unique, high quality, and cost effective services that enable each Project to effectively use in vivo models to probe various aspects of normal and tumor biology.