High Throughput Tools for Mammilian CellCulture Bioprocessing and Analysis

该项目寻求设计和建立一个灵活的机器人平台，以自动孵化和处理数百个在摇瓶中生长的哺乳动物细胞培养物，并集成细胞生长和代谢物分析。摇瓶模型规模较大的生物反应器，有足够的体积用于分析和支持过程开发和细胞系稳定性研究的频繁采样。目标是确保高通量生物过程研究的一致、可重复的细胞处理。这将有助于对影响细胞生长或生产力的多个参数进行系统、并行的研究，例如，培养基配方或饲喂制度。将开发“智能”软件来模拟过程并模仿培养专家，从而自动决定如何以及何时对每个单独的培养进行处理，例如，继代培养到目标细胞数，或何时喂食以维持设定的血糖水平。

The project seeks to design and build a flexible robotic platform to automate incubation and processing of hundreds of mammalian cell cultures growing in shake flasks, with integrated cell growth and metabolite analysis. Shake flasks model larger scale bioreactors, have ample volume for frequent sampling for analysis and support process development and cell line stability studies.The goal is to ensure consistent, reproducible cell processing for high throughput bioprocess research. This would facilitate systematic, parallel investigation of multiple parameters influencing cell growth or productivity, e.g. media formulation or feed regime."Smart" software would be developed to model the process and mimic the culture expert, and thus automatically decide how and when to process each individual culture e.g. subculture to a target cell count, or when to feed to maintain a set glucose level.