CORE--CELL AND ISLET ISOLATION CORE

拟议的细胞和胰岛分离核心(CIIC)将由两个实验室组成：细胞和胰岛分离实验室(CIL)和胰岛功能实验室(IFL)。CIIC的四个总体目标是：a)以经济、优质的方式为DERC成员分离脂肪细胞、选定的肌肉和胰岛，供DERC成员使用；b)为DERC成员提供胰岛功能评估、标准化和开发；C)以成本效益的方式为DERC成员提供培养液、胎牛血清和细胞培养和储存设施；以及D)鼓励DERC研究人员利用CIIC核心资源开展合作研究活动。DERC研究人员经常需要新分离的胰岛素靶组织和胰岛制剂，以测试遗传、药物、营养或行为干预的影响。然而，当这一点 使用是间歇性的，由个别研究项目决定，实验室无法保持分离这些组织和细胞的技术专长或设备。这对于胰岛组织来说尤其是一个问题。CIL将分离细胞和胰岛。杨赞东博士在胰岛分离方法和胰岛功能研究方面拥有丰富的经验。他和纳德勒博士将为这一核心提供总体指导。这个细胞和胰岛分离实验室将与Keller博士(动物特性核心主任)密切互动，进行特定的分析以及(胰岛素、C-肽)以及肌肉和脂肪细胞分离的其他技术专长。中心将利用设备齐全的设施，及时提供高质量的细胞、肌肉和胰岛，以满足这些研究的需求。杨博士和孟晨博士将在J·纳德勒博士的监督下领导IFL。本实验的目标是以标准化和经济高效的方式评估胰岛功能。IFL将评估由CIL分离的胰岛的功能，并分析β细胞和β1样细胞(如干细胞或其他分化的细胞 用于胰岛素分泌功能)。该实验室将进行A)胰岛和β细胞的动力学研究：静态胰岛素分泌和胰岛灌流；(B)胰岛的免疫组织化学研究，包括胰岛素、胰升糖素；胰岛和β细胞的细胞凋亡检测；以及图像文件服务。

The proposed Cell and Islet Isolation Core (CIIC) will be comprised of two laboratories: a cell and islet isolation laboratory (CIL) and islet function laboratory (IFL). The four general goals for the CIIC are: A) to isolate adipocytes, selected muscles and pancreatic islets from experimental animals, including rats, mice and pigs, for DERC members in an economical and high quality manner; B) to provide, standardize and develop assessments of islet function for the DERC members, C) to provide, in a cost efficient manner, culture media, fetal calf serum and cell culture and storage facilities for DERC members; and D) to encourage collaborative research activities among DERC investigators utilizing the resources of the CIIC core. DERC investigators frequently need freshly isolated preparations of insulin target tissues and pancreatic islets to test the impact of genetic, pharmacologic, nutritional or behavioral interventions. However, when this usage is intermittent and dictated by individual research projects, laboratories cannot maintain the technical expertise or equipment to isolate these tissues and cells. This is particularly an issue for islet tissue. The CIL will isolate cells and islets. Dr. Zandong Yang has extensive experience in methods for isolating islets and in the study of islet function. He and Dr. Nadler will provide overall direction to this Core. This Cell and Islet isolation lab will interact closely with Dr. Keller (Animal Characterization Core Director) both for the conduct of specific assays as well (insulin, c-peptide) as for additional technical expertise for muscle and adipocyte isolation. The CIIC will provide high-quality cells, muscles and islets in a timely manner using fully equipped facility to meet the demands of these studies. Dr. Yang with Dr. Meng Chen will direct the IFL, with oversight by Dr. J. Nadler. The goal of this lab is to assess islet function in a standardized and cost-efficient manner. The IFL will assess function of islets that are isolated by the CIL, and also analyze beta cells and beta-1ike cells (such as stem cells or other cells differentiated for insulin secretory function). This lab will perform A) kinetic studies in islets and beta cells: static insulin secretion and islet perifusion; (B) immunohistochemical studies of islets, including insulin, glucagon; apoptosis detection in islets and beta cells; and an image documentation service.