Differences in the Reaction Cycle among PMCA Isoforms

PMCA异构体反应周期的差异

• 批准号: 7185130
• 负责人: EMANUEL Ernst STREHLER
• 金额: $ 3.25万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7185130
• 关键词: ATP Hydrolysis; ATP phosphohydrolase; Affect; Alternative Splicing; Argentina; Binding; Ca(2+)-Transporting ATPase; Calcium; Calmodulin; Cations; Cell membrane; Cells; Clinic; Collaborations; Condition; Coupling; Cytoplasm; Erythrocyte Membrane; Eukaryotic Cell; Genes; Goals; Grant; Hydrolysis; Insecta; Ion Transport; Ions; Kinetics; Laboratories; Ligands; Mammals; Measures; Membrane; Memory; Modeling; Molecular Biology; Nucleic Acid Regulatory Sequences; Nucleotides; Physiological; Property; Protein Isoforms; Pump; Purpose; Rate; Reaction; Recording of previous events; Regulation; Research; Scheme; Side; Structure; Tissues; United States National Institutes of Health; Universities; Variant; cell type; member; mutant; parent grant

DESCRIPTION (provided by applicant): The plasma membrane calcium pump (PMCA) is one of the essential mechanisms to extrude calcium across the plasma membrane, thereby keeping and restoring a low cytosolic calcium concentration. In mammals, four genes encode separate isoforms of this pump, and this variability is further enhanced by alternative splicing. In total, there are more than 20 variants of the PMCA. These PMCA isoforms are differentially expressed in various tissues where they participate in cell type-specific calcium regulation. As is the case in many other cation-transporting ATPases, the reaction cycle of the PMCA includes the synthesis and hydrolysis of a phosphorylated intermediate. However, little is known about differences in the reaction cycle among different PMCAs. Also, during cation transport across the membrane, it is possible to distinguish an intermediate step in which the transporting ion is not accessible to either side of the membrane. This phenomenon is known as ion occlusion, and so far has not been characterized in the PMCA. To characterize the differences in the reaction cycle among different PMCAs, including the analysis of calcium occlusion, it is necessary to combine expertise both in the molecular biology of PMCAs and in the kinetic analysis of their reaction cycle. The proposed collaboration between the laboratory of Dr. Emanuel E. Strehler at the Mayo Clinic in Rochester, MN, and the laboratory of Dr. Juan P. Rossi at the University of Buenos Aires in Argentina provides a unique opportunity to study this important topic. This research will be done as an extension of NIH grant # RO1GM28835.

描述（由申请人提供）：

项目成果

Differential effects of G- and F-actin on the plasma membrane calcium pump activity.

• DOI: 10.1007/s12013-012-9467-6
• 发表时间: 2013-05
• 期刊: CELL BIOCHEMISTRY AND BIOPHYSICS
• 影响因子: 2.6
• 作者: Vanagas, Laura;Candelaria de La Fuente, Maria;Dalghi, Marianela;Ferreira-Gomes, Mariela;Rossi, Rolando C.;Strehler, Emanuel E.;Mangialavori, Irene C.;Rossi, Juan P. F. C.
• 通讯作者: Rossi, Juan P. F. C.

Diving Into the Lipid Bilayer to Investigate the Transmembrane Organization and Conformational State Transitions of P-type Ion ATPases.

深入研究脂质双层，研究 P 型离子 ATP 酶的跨膜组织和构象状态转变。

• DOI: 10.2174/187231311795243319
• 发表时间: 2011
• 期刊: Current chemical biology
• 作者: Mangialavori,IreneC;Caride,ArielJ;Rossi,RolandoC;Rossi,JuanPabloFC;Strehler,EmanuelE
• 通讯作者: Strehler,EmanuelE

Plasma membrane calcium pump (PMCA) differential exposure of hydrophobic domains after calmodulin and phosphatidic acid activation.

钙调蛋白和磷脂酸激活后，质膜钙泵 (PMCA) 疏水域的差异暴露。

• DOI: 10.1074/jbc.m110.210088
• 发表时间: 2011
• 期刊: The Journal of biological chemistry
• 作者: Mangialavori,Irene;Villamil-Giraldo,AnaMaría;Pignataro,MaríaF;Ferreira-Gomes,Mariela;Caride,ArielJ;Rossi,JuanPabloFC
• 通讯作者: Rossi,JuanPabloFC

Autoinhibition mechanism of the plasma membrane calcium pump isoforms 2 and 4 studied through lipid-protein interaction.

通过脂质-蛋白质相互作用研究质膜钙泵亚型 2 和 4 的自抑制机制。

• DOI: 10.1042/bj20111035
• 发表时间: 2012
• 期刊: The Biochemical journal
• 作者: Mangialavori,IreneC;Corradi,Gerardo;Rinaldi,DeboraE;delaFuente,MariaCandelaria;Adamo,HugoP;Rossi,JuanPabloFC
• 通讯作者: Rossi,JuanPabloFC