Compositional Analysis of the Arabidopsis 26S Proteasome

拟南芥 26S 蛋白酶体的组成分析

• 批准号: 7168202
• 负责人: ADAM J BOOK
• 金额: $ 4.47万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7168202
• 关键词: 26S proteasome; Agriculture; Amino Acids; Arabidopsis; Arabidopsis Proteins; Binding; Complex; Defect; Depth; Development; Disease; Endopeptidases; Eukaryota; Eukaryotic Cell; Excision; Genes; Goals; Hormonal; Individual; Knowledge; Lead; Localized; Maintenance; Medicine; Methods; Modeling; Molecular; Mouse-ear Cress; Organism; Pathway interactions; Peptide Hydrolases; Peptides; Physiological; Physiology; Plants; Protein Isoforms; Proteins; Research; Role; Signal Transduction; Substrate Specificity; System; Testing; Tissues; Ubiquitin; Variant; Work; Yeasts; feeding; genetic regulatory protein; in vivo; multicatalytic endopeptidase complex; protein degradation; response

DESCRIPTION (provided by applicant): The goal of this research is to reveal the molecular composition and functions of the 26S proteasome in a multicellular eukaryote, using Arabidopsis thaliana as the model. Organisms rely on protein degradation for a variety of essential functions, including the maintenance of free amino acid pools, the removal of damaged proteins, and the control of regulatory protein abundance. The major machine for this breakdown is the 26S proteasome, an ATP-dependent 2-MDa proteolytic complex. The specific aim of this project is to define the composition of the native 26S proteasome from Arabidopsis, reveal the functions of various subunits, and determine whether the plant preferentially assembles specific subtypes of the complex through selective pairing of various isoforms. A more complete characterization of the 26S proteasome will lead to a deeper understanding of how proteins are selectively degraded which may potentially identify new ways to control protein breakdown in vivo. Given the importance of Ub and the 26S proteasome to almost all facets of eukaryotic physiology and development, this work should have practical benefits to both medicine and agriculture, including various disease caused by defects in the Ubiquitin/26S proteasome system.

描述（申请人提供）：本研究的目的是以拟南芥为模型，揭示多细胞真核生物中26S蛋白酶体的分子组成和功能。生物体依靠蛋白质降解来实现各种基本功能，包括维持游离氨基酸库、去除受损蛋白质以及控制调节蛋白质丰度。这种分解的主要机制是 26S 蛋白酶体，一种 ATP 依赖性 2-MDa 蛋白水解复合物。该项目的具体目标是确定拟南芥天然26S蛋白酶体的组成，揭示各种亚基的功能，并确定植物是否通过选择性配对各种亚型来优先组装复合物的特定亚型。对 26S 蛋白酶体进行更完整的表征将有助于更深入地了解蛋白质如何选择性降解，这可能会找到控制体内蛋白质分解的新方法。鉴于Ub和26S蛋白酶体对真核生理学和发育的几乎所有方面的重要性，这项工作应该对医学和农业都有实际益处，包括由泛素/26S蛋白酶体系统缺陷引起的各种疾病。