Improved Preservation of Suspended Cells

Cell Preservation Services，Inc.（CPSI）致力于开发生物技术和医学领域的新工艺和设备。 CPSI的研究策略侧重于理解和操纵细胞存活和细胞死亡途径，以应对各种压力。最近，我们已经认识到，这是不够的，仅仅解决在孤立的事件的细胞加工过程中的应激反应问题，在过去一直是焦点，但事实上，一个更全面的和涉及过程集成和细胞调节的策略是必要的。CPSI打算应用这种新的战略方法来开发新的方法，以提高人类肺细胞（hLC）和组织的质量（完整性和功能），超越目前通常实现的水平。 在本研究项目下进行的调查将利用cDNA微阵列，蛋白质组学分析，以及活力和功能分析，以确定在常见的移植前操作和处理过程中激活的hLC的应激反应途径。 这些研究的目的是更好地理解hLC对离体操作的独特反应，并利用这些数据开发用于细胞处理的新方法。 这些发展不仅对肺组织样本的质量和可用性产生影响，而且还能够在体外研究以及细胞和组织移植等领域扩大利用。

Cell Preservation Services, Inc. (CPSI) is dedicated to the development of novel processes and devices in the area of biotechnology and medicine. CPSI research strategy focuses on understanding and manipulating the cell survival and cell death pathways activated in response to various stressors. Recently, we have recognized that it is not adequate to merely address stress response issues during isolated events of cell processing, as has been the focus in the past, but in fact a more comprehensive and strategy involving process integration and cellular conditioning is necessary. CPSI intends to apply this novel strategic approach to develop new methodologies to improve the quality (integrity and function) of human lung cells (hLC) and tissues beyond those typically realized today. Investigations conducted under this research project will utilize cDNA microarrays, proteomic profiling, and viability and functional analysis to define the stress response pathways activated in hLC during common pre-transplant manipulation and processing. It is the intent of these studies to provide a better understanding of the unique responses of hLC to ex vivo manipulation and utilize these data to develop new methodologies for cell processing. These developments should have an impact not only on the quality and availability of lung tissue samples, but also enable expanded utilization in areas such as in vitro study as well cell and tissue transplants.