Improved Preservation of Suspended Cells

细胞保存服务公司(CPSI)致力于生物技术和医学领域的新工艺和新设备的开发。CPSI的研究策略侧重于理解和操纵细胞生存和细胞死亡途径，以响应各种应激源。最近，我们认识到，仅仅像过去关注的那样，在细胞处理的个别事件中解决应激反应问题是不够的，但实际上需要一种更全面的、涉及过程整合和细胞调节的策略。CPSI打算应用这一新的战略方法来开发新的方法，以提高人类肺细胞(HLC)和组织的质量(完整性和功能)，而不是今天通常实现的质量。在这项研究项目下进行的研究将利用基因芯片、蛋白质组图谱以及活性和功能分析来确定在常见的移植前操作和处理过程中HLC中激活的应激反应通路。这些研究的目的是为了更好地了解HLC对体外操作的独特反应，并利用这些数据开发新的细胞处理方法。这些进展不仅应该对肺组织样本的质量和可获得性产生影响，而且还可以扩大在体外研究以及细胞和组织移植等领域的利用。

Cell Preservation Services, Inc. (CPSI) is dedicated to the development of novel processes and devices in the area of biotechnology and medicine. CPSI research strategy focuses on understanding and manipulating the cell survival and cell death pathways activated in response to various stressors. Recently, we have recognized that it is not adequate to merely address stress response issues during isolated events of cell processing, as has been the focus in the past, but in fact a more comprehensive and strategy involving process integration and cellular conditioning is necessary. CPSI intends to apply this novel strategic approach to develop new methodologies to improve the quality (integrity and function) of human lung cells (hLC) and tissues beyond those typically realized today. Investigations conducted under this research project will utilize cDNA microarrays, proteomic profiling, and viability and functional analysis to define the stress response pathways activated in hLC during common pre-transplant manipulation and processing. It is the intent of these studies to provide a better understanding of the unique responses of hLC to ex vivo manipulation and utilize these data to develop new methodologies for cell processing. These developments should have an impact not only on the quality and availability of lung tissue samples, but also enable expanded utilization in areas such as in vitro study as well cell and tissue transplants.