Nanostructured substrates for cell assays

用于细胞分析的纳米结构基质

• 批准号: 7282394
• 负责人: Renee L Herber
• 金额: $ 48.65万
• 依托单位: PLATYPUS TECHNOLOGIES, LLC
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7282394
• 关键词: Address; Antibodies; Biological Assay; Cell Count; Cell membrane; Cells; Cellular Membrane; Class; Compatible; Development; Dyes; Elements; Enzymes; Fluorochrome; Goals; Label; Lipids; Location; Masks; Methods; Migration Assay; Optics; Phase; Polarization Microscopy; Protein Binding; Reader; Reporting; Research Project Grants; Small Business Funding Mechanisms; Small Business Innovation Research Grant; System; Technology; Time; To specify; base; cell motility; cost; cost effective; innovation; liquid crystal; nanostructured; novel; response; technology development

DESCRIPTION (provided by applicant): Our goal in this Phase 2 SBIR proposal is to develop and optimize the components for a novel cell migration assay. In Phase 1, we demonstrated that liquid crystals assume a predictable orientation in response to cellular membranes, and therefore they could be used in a sensitive, rapid, generalizable method for reporting cell number. In this application, we propose to build on the ability of liquid crystals to accurately report cell number and spatial location in the development of a quantitative assay for cell migration. This innovative approach will provide a technology with many intrinsic advantages over current methods by reducing labor, time and cost and by providing compatibility with high throughput formats.

描述（由申请人提供）：我们在这个2期SBIR提案中的目标是开发和优化用于新型细胞迁移试验的成分。在第一阶段，我们证明了液晶在响应细胞膜时具有可预测的方向，因此它们可以用于灵敏，快速，通用的报告细胞数量的方法。在这个应用中，我们建议建立在液晶准确报告细胞数量和空间位置的能力上，以开发细胞迁移的定量分析。这种创新的方法通过减少劳动力、时间和成本以及提供与高吞吐量格式的兼容性，将提供一种比当前方法具有许多内在优势的技术。