Addressable Digital Array Maps: Mapping Human Plasma with 2D Ion Mobility, Mass S

可寻址数字阵列图：利用 2D 离子淌度（质量 S）绘制人体血浆图

• 批准号: 7272343
• 负责人: Stephen Valentine
• 金额: $ 14.67万
• 依托单位: PREDICTIVE PHYSIOLOGY AND MEDICINE, INC.
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-03 至 2008-04-01
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7272343
• 关键词: Address; Biological Markers; Biology; Businesses; Cardiovascular Diseases; Cataloging; Catalogs; Coronary Arteriosclerosis; Data; Data Analyses; Databases; Detection; Development; Disease; Endopeptidases; Foundations; Future; Gases; Generations; Genomics; Health; Hour; Human; Individual; Ions; Liquid Chromatography; Maps; Mass Spectrum Analysis; Measurement; Medicine; Methods; Modeling; Molecular Weight; Numbers; Output; Patients; Pattern; Peptide Hydrolases; Phase; Phase II Clinical Trials; Physiology; Plasma; Population; Predisposition; Preventive Medicine; Proteins; Proteome; Proteomics; Relative (related person); Research; Resolution; Role; Sampling; Score; Spectrometry; System; Systems Biology; Techniques; Technology; Time; Tube; Work; cohort; comparative; design; digital; expectation; falls; high throughput analysis; improved; instrument; instrumentation; ion mobility; ion source; liquid chromatography mass spectrometry; mass spectrometer; medical schools; metabolomics; protein metabolite; research study

DESCRIPTION (provided by applicant): A new proteomics technology utilizing multidimensional (2D) ion mobility spectrometry separation steps combined with mass spectrometry will be developed to characterize the human plasma proteome. The new instrumentation provides sufficient peak capacity such that a high-throughput analysis of the plasma proteome can be performed without the need for a LC separation step. Thus the measurement can be performed on a timescale that is 30 times faster. The instrumentation will be used to generate Addressable Digital Array Maps (ADAMs) for human plasma analyses. These searchable databases will be utilized in comparative proteomics studies (Phase II) to create a cardiovascular disease ADAM (map). The rapid, high-throughput measurement provided by the 2D IMS instrumentation is groundbreaking in that it is now possible to perform plasma profiling studies on a timescale necessary for population proteomics studies. Such studies are required to identify and score multiple biomarkers to populate the cardiovascular disease ADAM. In the future the map will be used to provide an accurate assessment of health for customers in our personalized medicine business model. The research described in this proposal involves the development of instrumentation required to perform population proteomics studies in a timely fashion. These studies will allow the development of disease biomarker databases that can be used in assessments of disease predisposition for our personalized medicine business model.

描述(申请人提供)：将开发一种新的蛋白质组学技术，利用多维(2D)离子迁移率光谱分离步骤与质谱学相结合来表征人血浆蛋白质组。新的仪器提供了足够的峰容量，因此可以在不需要LC分离步骤的情况下执行血浆蛋白质组的高通量分析。因此，可以在快30倍的时间刻度上执行测量。该仪器将用于生成用于人体血浆分析的可寻址数字阵列地图(ADAMS)。这些可搜索的数据库将用于比较蛋白质组学研究(第二阶段)，以创建心血管疾病ADAM(MAP)。2D IMS仪器提供的快速、高通量测量具有开创性，因为现在可以在群体蛋白质组研究所需的时间尺度上进行血浆图谱研究。这样的研究需要识别和评分多个生物标记物来填充心血管疾病ADAM。未来，该地图将用于在我们的个性化医疗商业模式中为客户提供准确的健康评估。这项建议中描述的研究涉及开发及时进行种群蛋白质组学研究所需的仪器。这些研究将使疾病生物标志物数据库的开发能够用于我们个性化医疗商业模式的疾病易感性评估。