ACQUISITION OF A 500MHZ HIGH-RESOLUTION NMR CRYOPROBE

采集 500MHZ 高分辨率 NMR 冷冻探针

• 批准号: 7335135
• 负责人: OM PRAKASH
• 金额: $ 14.1万
• 依托单位: KANSAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-10 至 2007-07-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335135
• 关键词: aging; computer program /software; computer system hardware; computers; cryoscience; lead; performance; solubility; solutions; success; suppression; university; water

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. The Kansas State University High Resolution NMR Facility, which has served more than 23 separate NIH funded research projects and several other life science research groups (total 40), requests funding to upgrade an existing Varian UNITYplus 500 MHz instrument (purchased in 1993) with a UNITYINOVA digital console and to purchase an additional high sensitivity 'state-of-the-art' 5 mm triple resonance inverse detection cryo-probe. This acquisition will allow the research programs of nine major users and many other NIH supported investigators to take advantage of revolutionary cryogenic probe technology that is becoming essential for studying solution structure and dynamics of large biomolecules and other compounds that are currently inaccessible due to inherent weakness of NMR signal, short-lived stability, limited solubility, or low sample availability. The cryo-probe system will allow NMR data to be acquired with a sensitivity that is 3 to 4- fold greater than our current probe. The UNITYINOVA digital console is required for the proper installation and function of the cryo-probe assembly. The console upgrade request is also justified by a growing number of deficiencies of our current NMR system. Among these are the following: 1) outdated NMR acquisition computer module leading to difficulty in data acquisition, 2) radio-frequency phase instability resulting in inadequate water suppression and diminished performance in multi-dimensional experiments, 3) lock channel signal instability during long hours experiments, 4) extensive down time due to ever-increasing host to acquisition board linking failure, and 5) extreme difficulty in implementing routine software revision and computer hardware improvements due to the age of the console. The funding of this project will provide a shared instrumentation necessary to complete more than ten on going NIH supported research programs at KSU and to elevate inter-institutional bio-medical research collaborations between KSU NMR facility and nearby institutions.

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。堪萨斯州立大学高分辨率核磁共振设备，为超过23个独立的NIH资助的研究项目和其他几个生命科学研究小组提供服务（共40人），申请资金升级现有的Varian UNITYplus 500 MHz仪器（1993年购买）与UNITYINOVA数字控制台，并购买额外的高灵敏度“国家的，art' 5 mm三重共振反向检测冷冻探针。此次收购将使九个主要用户和许多其他NIH支持的研究人员的研究计划能够利用革命性的低温探针技术，该技术对于研究大生物分子和其他化合物的溶液结构和动力学变得至关重要，这些化合物目前由于NMR信号的固有弱点，短期稳定性，有限的溶解度或低样品可用性而无法获得。冷冻探针系统将允许核磁共振数据采集的灵敏度是我们目前探针的3至4倍。UNITYINOVA数字控制台是冷冻探针组件正确安装和运行所必需的。控制台升级的要求也是合理的，越来越多的缺陷，我们目前的核磁共振系统。其中包括：1）过时的NMR采集计算机模块导致数据采集困难，2）射频相位不稳定性导致水抑制不足和多维实验中的性能降低，3）长时间实验期间锁定通道信号不稳定，4）由于主机到采集板链接故障不断增加而导致的长时间停机，以及5）由于控制台的年龄，在实现例行软件修订和计算机硬件改进方面极其困难。 该项目的资金将提供必要的共享仪器，以完成KSU十多个正在进行的NIH支持的研究项目，并提升KSU NMR设施和附近机构之间的机构间生物医学研究合作。