Amersham Biosciences Typhoon 9410

描述（由申请人提供）：本申请用于购买阿默舍姆Biosciences Typhoon 9410。Typhoon将作为细胞生物学，微生物学和药理学部门的核心工具，拥有29名研究人员的用户组，几乎所有人都获得了NIH的资助，具有广泛的研究兴趣。此外，纽约大学医学院内任何其他需要使用Typhoon特殊功能的研究人员将被允许加入用户组。目前在整个医学院中没有类似的仪器。Typhoon 9410将磷光成像仪、荧光图像扫描仪和冷光阅读器结合在一台机器中。除了阿默舍姆，没有其他公司销售具有相同功能的仪器。此外，台风将取代目前使用的过时的分子动力学425磷光成像仪。由于用户组中的大多数实验室已经拥有只能由阿默舍姆仪器读取的分子动力学试剂卡，因此购买Typhoon是适当的，具有成本效益。台风还将取代老化和有限的分子动力学荧光计575，目前是在一个单独的建设。Typhoon荧光计比其前身灵敏得多，提供更好的分辨率，并且可以检测更广泛的荧光底物。Typhoon的检测能力不仅将提供改进的荧光测量，而且还将使用荧光探针标记的Western和核酸杂交印迹的定量成像成为可能，取代X射线胶片用于使用增强的化学发光检测印迹，并允许在一维和二维聚丙烯酰胺凝胶上用荧光染料染色的蛋白质的可视化和定量。还要求提供资金，用于购买软件许可证，以便在各部门建立数据分析工作站，与仪器所在设施分开。用户组的一个小型委员会将管理仪器并为其使用设计规则。训练有素的技术人员将每天维护和监控机器，并教育新用户。预计台风的可用性将对用户组和整个机构的研究产生重大影响。

DESCRIPTION (provided by applicant): This request is for the purchase of an Amersham Biosciences Typhoon 9410. The Typhoon will serve as a core instrument for the Departments of Cell Biology, Microbiology, and Pharmacology with a user group of 29 investigators, nearly all with NIH funding, with a wide variety of research interests. In addition, any other investigator within the NYU School of Medicine who requires the use of the special capabilities of the Typhoon will be allowed to join the user group. No similar instrument currently exists in the entire School of Medicine. The Typhoon 9410 combines a phosphorimager, a fluorescence image scanner, and a luminescence reader in one machine. No company other than Amersham markets an instrument with the same capabilities. Moreover, the Typhoon will replace an obsolete Molecular Dynamics 425 phosphorimager currently in use. Since most of the laboratories in the user group already own Molecular Dynamics cassettes, which can only be read by the Amersham instrument, the purchase of the Typhoon is appropriate and cost effective. The Typhoon will also supplant an aging and limited Molecular Dynamics Fluorimeter 575 that is currently housed in a separate building. The Typhoon fluorimeter is much more sensitive than its predecessor, offers better resolution, and can detect a wider variety of fluorescent substrates. The detection capabilities of the Typhoon will not only provide improved fluorimetric measurements but also will enable quantitative imaging of Western and nucleic acid hybridization blots labeled with fluorescent probes, replace X-ray film for detection of blots using enhanced chemiluminescence, and allow visualization and quantitation of proteins stained with fluorescent dyes on one- and two-dimensional polyacrylamide gels. Funds are also requested for a software license to set up workstations for data analysis in the individual departments separate from the facility housing the instrument. A small committee of the user group will administer the instrument and design rules for its use. A trained technician will maintain and monitor the machine on a daily basis and educate new users. It is anticipated that the availability of the Typhoon will have great impact on the research of the user group and the institution as a whole.