ARRAY

阵列核心提供用于分析DMA拷贝数变化(阵列)的阵列和相关服务 Cgh)以及定制阵列打印。 阵列CGH：用于CGH分析的阵列由分布在人和小鼠身上的袋状克隆组成 基因组。这些阵列允许可靠地检测来自二倍体和高水平的单拷贝变化 放大。袋子阵列从福尔马林固定石蜡包埋样本中产生良好的数据，因此 允许使用其他来源的数组进行目前不可能进行的研究。核心将继续提供 -1-1.5 Mb分辨率的袋子阵列，用于人和鼠的分析，以及32,000袋完整的人 基因组拼接阵列。后者允许分辨率达到袋子长度的一小部分，或远低于100kb。在……里面 除了这些标准数组外，核心还将为个别项目打印定制数组，提供说明 关于它提供的袋子阵列的使用(DMA分离、标记、杂交、成像和分析)，并咨询 关于数据的解释。它还将继续提供全阵列处理服务，从 调查人员和返回的数据。 仪器设备：Core运行三个阵列成像系统，一台Axon 4000B激光扫描仪，一台定制 构建了1M像素的CCD摄像系统，以及定制的4M像素的CCD成像系统。核心也是 操作定制的高速阵列打印机。其他设备包括一个液体搬运机器人和一个完整的 用于阵列处理和印刷溶液制备的库存实验室。

The Array Core provides arrays and associated services for analysis of DMA copy number variation (array CGH) as well as custom array printing. Array CGH: The arrays for CGH analysis consist of BAG clones distributed over the human^and mouse genomes. These arrays allow reliable detection of single copy changes from diploid as well as high-level amplifications. The BAG arrays produce good data from formalin fixed-paraffin embedded samples and thus allow studies that are not currently possible with arrays from other sources. The core will continue to offer BAG arrays with -1-1.5 Mb resolution for human and murine analysis, and a 32,000 BAG whole human genome tiling array. The latter allows resolution to a fraction of the length of a BAG, or well below 100kb. In addition to these standard arrays, the Core will print custom arrays for individual projects, provide instruction on use of the BAG arrays it offers (DMA isolation, labeling, hybridization, imaging and analysis), and consult on interpretation of data. It will also continue to offer a full array processing service, receiving DNA from investigators and returning data. Instrumentation: The Core operates three array imaging systems, an Axon 4000B laser scanner, a custom built 1M pixel CCD camera system, and a custom built 4M pixel CCD imaging system. The Core also operates a custom, high-speed array printer. Other equipment includes a liquid handling robot and a fully stocked laboratory for array processing and preparation of printing solutions.