IMMUNOHISTOCHEMISTRY AND MOLECULAR PATHOLOGY

免疫组织化学和分子病理学核心的目标是促进发展和 免疫组织化学和分子遗传学分析在癌症中心成员中的应用。 核心意志：‘’^ 1.建立基于免疫组化和原位杂交(FISH)的新抗体和遗传位点检测方法 与癌症中心成员合作 2.对动物和人体组织切片和细胞系进行常规免疫组织化学分析。 3.对癌症中心组织核心生产的组织阵列进行IHC分析。 4.对细胞系、动物模型和人类肿瘤中的DNA拷贝数进行FISH分析。 5.与CC成员合作进行显微解剖，从肿瘤切片中提取DNA和RNA。 6.对人和小鼠DNA克隆的中期染色体进行基因组作图 7.进行人和小鼠的比较基因组杂交 核心将与其他核心(特别是组织、基因组分析和阵列核心)密切合作，以 允许研究方案利用每个核心中提供的资源和专业知识。

The goal of the Immunohistochemistry and Molecular Pathology Core is to facilitate development and application of immunohistochemical and molecular genetic analyses for Cancer Center members. The Core will: ' ' ^ 1. Develop IHC and in situ hybridization (FISH) based assays for new antibodies and genetic loci in collaboration with CancerCenter Members 2. Perform routine immunohistochemical analyses of animal and human tissue sections and cell lines. 3. Perform IHC analysis of tissue arrays produced by the Cancer Center Tissue Core. 4. Perform FISH analyses for DMA copy number in cell lines, animal models, and human tumors. 5. Collaborate with CC Members in microdissection, and extraction of DNA and RNA from tumor sections. 6. Perform genomic mapping onto metaphase chromosomes of human and mouse DNA clones 7. Perform human and mouse comparative genomic hybridization The Core will work closely with other Cores (particularly the Tissue, Genome Analysis, and Array Cores) to allow research protocols to take advantage of the resources and expertise offered within each Core.