ANIMAL MODEL CORE

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。目的：本计划项目对HIV潜伏期和病毒库的机制进行全面分析。组织培养系统中的体外研究（项目1、2和3）将与该核心中的体内研究（“非人灵长类动物的HIV/SIV储库和潜伏期”）密切协调。因此，该核心将为本计划项目中的动物模型研究提供恒河猴和适当的基础设施支持。三个具体目的解决了这一假设：目的1：定义SIV/猕猴和RT-SHIV/猕猴系统中的条件，以分析在感染HIV的人中观察到的病毒潜伏期/储库;目的2：分析接受抗逆转录病毒治疗的感染猕猴中的病毒定位;目的3：确定病毒和宿主细胞因子在调节病毒潜伏期、持续性和再活化中的作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: This Program Project conducts a comprehensive analysis of the mechanism(s) of HIV latency and viral reservoirs. In vitro investigations in tissue culture systems (Projects 1, 2, and 3) will be closely coordinated with in vivo studies in this core ("Non-human Primates for HIV/SIV Reservoirs and Latency"). Accordingly, this core will provide rhesus macaques and appropriate infrastructure support required for the animal model studies in this Program Project. Three Specific Aims address this hypothesis: Aim 1: to define conditions in the SIV/macaque and RT-SHIV/macaque systems for analysis of viral latency/reservoirs as observed in humans infected with HIV; Aim 2: to analyze virus localization in infected macaques undergoing antiretroviral therapy; Aim 3: to determine the role of viral and host-cell factors in the regulation of viral latency, persistence, and reactivation.