ANIMAL MODEL CORE

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。目的：本课题对艾滋病病毒潜伏期和病毒库的发病机制(S)进行了全面的分析。组织培养系统的体外研究(项目1、2和3)将与这一核心(“艾滋病毒/SIV储存库和潜伏期的非人类灵长类动物”)的体内研究密切协调。因此，该核心将提供恒河猴和本计划项目中动物模型研究所需的适当基础设施支持。针对这一假设有三个具体的目的：目的1：确定SIV/Macaque和RT-Shiv/Macaque系统中的条件，用于分析在感染艾滋病毒的人类中观察到的病毒潜伏期/储存库；目标2：分析病毒在接受抗逆转录病毒治疗的感染猕猴中的定位；目标3：确定病毒和宿主细胞因素在调节病毒潜伏期、持久性和重新激活中的作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: This Program Project conducts a comprehensive analysis of the mechanism(s) of HIV latency and viral reservoirs. In vitro investigations in tissue culture systems (Projects 1, 2, and 3) will be closely coordinated with in vivo studies in this core ("Non-human Primates for HIV/SIV Reservoirs and Latency"). Accordingly, this core will provide rhesus macaques and appropriate infrastructure support required for the animal model studies in this Program Project. Three Specific Aims address this hypothesis: Aim 1: to define conditions in the SIV/macaque and RT-SHIV/macaque systems for analysis of viral latency/reservoirs as observed in humans infected with HIV; Aim 2: to analyze virus localization in infected macaques undergoing antiretroviral therapy; Aim 3: to determine the role of viral and host-cell factors in the regulation of viral latency, persistence, and reactivation.