FASTEEM II: SPECTROFLUOROMETER FOR REAL TIME DIAGNOSIS OF DISEASE

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。我们已经开发了FastEEM II，这是第二代便携式荧光仪，用于实时、活体诊断疾病。该系统采集反射率(300？800 nm)和激光诱导荧光(10个激发波长，308？480 nm)光谱，采集时间不到一秒(0.3 S)。光的传输和收集是通过光纤探头进行的，使该设备与许多内窥镜手术兼容。我们利用国家仪器公司的LabVIEW开发了控制仪器的软件。该软件允许合并光谱分析算法(正在单独开发)，这将使FastEEM能够提供实时诊断。新的电子学和光学布局增加了该系统临床使用的可靠性。我们目前正在努力缩小该系统的尺寸并提高其易用性，以使其更符合临床环境。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have developed the FastEEM II, a second generation portable spectrofluorometer for the real-time, in vivo diagnosis of disease. This system collects reflectance (300 ¿ 800 nm) and laser induced fluorescence (10 excitation wavelengths, 308 ¿ 480 nm) spectra in a fraction of a second (0.3 s). Light delivery and collection is via a fiber optic probe, making the device compatible with many endoscopic procedures. We have developed software using National instruments LabView to control the instrumentation. This software allows for the incorporation of spectral analysis algorithms (being developed separately), which will enable the FastEEM to provide a real-time diagnosis. New electronics and optical layout have increased reliability of this system for clinical use. We are currently working to reduce the size and improve the ¿ease-of-use¿ of this system to make it more compatible with the clinical environment.