FLASH/REASH LABELING OF TETRACYSTEINE-TAGGED PROTEINS IN ZEBRAFISH

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。这个项目的目标是熟悉标记斑马鱼胚胎的技术方面，这些斑马鱼胚胎表达的蛋白质经过基因改造，含有四半胱氨酸标签。该标签被商业上可获得的荧光团Flash-EDT2和ReAsH-EDT2所识别。任何一个荧光团与四半胱氨酸标记物的结合都会产生荧光信号，该信号可以将二氨基联苯胺光转化为嗜奥斯酸的沉淀物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this project is to become familiar with the technical aspects of labeling zebrafish embryos that express proteins genetically altered to contain a tetracysteine tag. This tag is recognized by the commercially available fluorophores FlAsH-EDT2 and ReAsH-EDT2. Binding of either fluorophore to the tetracysteine tag results in a fluorescent signal that can photoconvert diaminobenzidine into an osmiophilic precipitate.