LENTIVIRUS HOST INTERACTIONS DURING ASSEMBLY & RELEASE: HIV

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. HIV-1 assembly and release can be visualized in live cells through the usage of fluorescent protein (FP) tagged viral structural proteins. While FP-tagged virus-like particles faithfully mimic viral morphogenesis, such virus is not replication-competent. We hypothesize however, that replication-competent HIV-1 will tolerate the small genetic tag that will allow labeling the viral structural proteins with FlAsH. Proviruses carrying the optimized version of this tag will thus be constructed and the distribution of the tagged virus will be compared to the location of non-tagged virus in doubly infected/transfected cells. If the two viruses co-localize, we will use the tagged version to analyze HIV assembly and its transmission from cell-to-cell.