K+ SYMPORTER

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们带来了一种膜蛋白的晶体，它可以共同运输K+和Na+。目前还没有公开的Na/K-共转运体的结构，这种结构将揭示离子共转运的机制。最近，我们在国际象棋测试晶体，我们发现了一些晶体的条件，使其达到~4A。我们一直在优化条件，需要访问以检查新晶体并收集完整的数据集。由于衍射图样的弱点，在国内收集数据已被证明是不可能的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are bringing crystals of a membrane protein that co-transports K+ and Na+. There are no published structures of Na/K-symporters and this structure would shed light into the mechanism of ion co-transport. Recently we were at CHess testing crystals and we found some conditions with crystals that diffract to ~4A. We have been optimizing conditions and need access to check the new crystals and collect full data sets. Data collection at home source has proved to be impossible due to do the weakness of the diffraction pattern.