NMR STUDY ON CUTDOMAIN DNA BINDING PROTEIN

剪切域DNA结合蛋白的核磁共振研究

• 批准号: 7420565
• 负责人: XIUBEI LIAO
• 金额: $ 0.26万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-20 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7420565
• 关键词: DNA; macromolecule; nucleic acids; proteins; solutions

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Structure and dynamics study of cutdomain protein using NMR. The cutdomain protein is a member of important family of DNA-binding proteins. Research in the field of macromolecule structure has progressed very rapidly. X-ray diffraction techniques have been successfully used to study the crystal structure of number of macromolecules, but methods to determine solution structures of macromolecules had previously been limited. Recent technical improvements in high resolution NMR spectroscopy combined with molecular biology techniques provide powerful tools to study the structure and dynamics of macromolecules. Since NMR is very sensitive to local conformational changes and gives direct information on the tertiary structure and dynamics of macromolecules in solution, it plays an increasingly important role in these studies. A focus of this laboratory involves studies of protein-nucleic acid interactions by a combination of NMR and biochemical techniques. NMR is used to study the structure and dynamics of both the free protein and the protein-nucleic acid complex. The biochemical techniques are used to relate the structure to its function.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。使用核磁共振研究剪切域蛋白的结构和动力学。剪切域蛋白是重要的 DNA 结合蛋白家族的成员。高分子结构领域的研究进展非常迅速。 X射线衍射技术已成功用于研究多种大分子的晶体结构，但以前确定大分子溶液结构的方法受到限制。最近高分辨率核磁共振波谱技术的进步与分子生物学技术相结合，为研究大分子的结构和动力学提供了强大的工具。由于核磁共振对局部构象变化非常敏感，并且可以直接提供有关溶液中大分子的三级结构和动力学的信息，因此它在这些研究中发挥着越来越重要的作用。该实验室的重点是通过核磁共振和生化技术相结合来研究蛋白质-核酸相互作用。 NMR 用于研究游离蛋白质和蛋白质-核酸复合物的结构和动力学。生化技术用于将结构与其功能联系起来。