NMR STUDY ON CUTDOMAIN DNA BINDING PROTEIN

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。核磁共振法研究切域蛋白的结构和动力学。切割结构域蛋白是DNA结合蛋白家族中的重要成员。高分子结构领域的研究进展非常迅速。X射线衍射法已被成功地用于研究许多大分子的晶体结构，但以前确定大分子溶液结构的方法一直受到限制。近年来，高分辨核磁共振波谱技术的进步与分子生物学技术的结合为研究大分子的结构和动力学提供了强有力的工具。由于核磁共振对局部构象变化非常敏感，并能直接提供溶液中大分子的三级结构和动力学信息，因此在这些研究中发挥着越来越重要的作用。这个实验室的一个重点是结合核磁共振和生化技术来研究蛋白质-核酸的相互作用。核磁共振被用来研究游离蛋白质和蛋白质-核酸复合体的结构和动力学。生化技术被用来将结构与其功能联系起来。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Structure and dynamics study of cutdomain protein using NMR. The cutdomain protein is a member of important family of DNA-binding proteins. Research in the field of macromolecule structure has progressed very rapidly. X-ray diffraction techniques have been successfully used to study the crystal structure of number of macromolecules, but methods to determine solution structures of macromolecules had previously been limited. Recent technical improvements in high resolution NMR spectroscopy combined with molecular biology techniques provide powerful tools to study the structure and dynamics of macromolecules. Since NMR is very sensitive to local conformational changes and gives direct information on the tertiary structure and dynamics of macromolecules in solution, it plays an increasingly important role in these studies. A focus of this laboratory involves studies of protein-nucleic acid interactions by a combination of NMR and biochemical techniques. NMR is used to study the structure and dynamics of both the free protein and the protein-nucleic acid complex. The biochemical techniques are used to relate the structure to its function.