NMR STUDY ON CUTDOMAIN DNA BINDING PROTEIN

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。剪切结构域蛋白质结构和动力学的NMR研究。切割结构域蛋白是DNA结合蛋白家族的重要成员。高分子结构领域的研究进展非常迅速。X射线衍射技术已成功地用于研究许多大分子的晶体结构，但以前确定大分子溶液结构的方法有限。近年来，高分辨核磁共振波谱技术与分子生物学技术相结合，为研究大分子的结构和动力学提供了强有力的工具。由于NMR对局部构象变化非常敏感，并能直接提供溶液中大分子的三级结构和动力学信息，因此在这些研究中发挥着越来越重要的作用。该实验室的一个重点是通过NMR和生物化学技术的结合研究蛋白质-核酸相互作用。NMR用于研究游离蛋白质和蛋白质-核酸复合物的结构和动力学。生物化学技术用于将结构与其功能联系起来。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Structure and dynamics study of cutdomain protein using NMR. The cutdomain protein is a member of important family of DNA-binding proteins. Research in the field of macromolecule structure has progressed very rapidly. X-ray diffraction techniques have been successfully used to study the crystal structure of number of macromolecules, but methods to determine solution structures of macromolecules had previously been limited. Recent technical improvements in high resolution NMR spectroscopy combined with molecular biology techniques provide powerful tools to study the structure and dynamics of macromolecules. Since NMR is very sensitive to local conformational changes and gives direct information on the tertiary structure and dynamics of macromolecules in solution, it plays an increasingly important role in these studies. A focus of this laboratory involves studies of protein-nucleic acid interactions by a combination of NMR and biochemical techniques. NMR is used to study the structure and dynamics of both the free protein and the protein-nucleic acid complex. The biochemical techniques are used to relate the structure to its function.