PROTEOMICS OF AXOPLASMIC SIGNALING COMPLEXES IN HEALTHY AND INJURED NERVES

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。这个合作项目涉及神经元细胞轴浆中信号复合体的蛋白质组学特征。目前正在研究两种类型的复合体，一种是对轴突的长距离通讯很重要的复合体，另一种是涉及局部反应的复合体。结合先进的质谱学方法的亲和蛋白质组学和差异蛋白质组学正在被采用。此外，正在开发质谱学方法来鉴定和建立神经元轴浆蛋白的新的翻译后修饰。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This collaborative project involves the proteomic characterization of signaling complexes in neuronal cell axoplasm. Two types of complexes are under investigation, those important for long distance communication along the axon, and those involved in localized responses. Both affinity and differential proteomics incorporating advanced mass spectrometry methods are being employed. In addition, mass spectrometric methods are being developed to identify and establish novel posttranslational modifications in neuronal axoplasm protei