PROTEOMICS OF AXOPLASMIC SIGNALING COMPLEXES IN HEALTHY AND INJURED NERVES

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。该合作项目涉及神经细胞轴质中信号复合物的蛋白质组学特征。目前正在研究的复合体有两种，一种是对沿轴突的长距离交流很重要的复合体，另一种是参与局部反应的复合体。结合先进的质谱分析方法的亲和和差异蛋白质组学正在被采用。此外，质谱方法正在发展，以确定和建立新的翻译后修饰在神经元轴质蛋白

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This collaborative project involves the proteomic characterization of signaling complexes in neuronal cell axoplasm. Two types of complexes are under investigation, those important for long distance communication along the axon, and those involved in localized responses. Both affinity and differential proteomics incorporating advanced mass spectrometry methods are being employed. In addition, mass spectrometric methods are being developed to identify and establish novel posttranslational modifications in neuronal axoplasm protei