MYOCELLULAR ENERGY METABOLISM: STUDIES USING SELF-REFERENCING MICROELECTRODES

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Dr Harper's studies focus on the molecular physiology of the muscle glucose transporter as it relates to Type II diabetes. Insufficient glucose sequestration within this tissues is accepted as one clear complication of the disease progression. Measuring glucose transport has previously been complicated and slow. However, the advent of the self-referencing glucose microsensor, developed by the BioCurrents Research Center, has greatly improved our ability to examine the dynamics of transport at the single cell level - the subject of the studies in Dr Harper's laboratory. To facilitate this research a full amperometric system was installed in Dr Harper's laboratory in Ottawa. The group is now fully capapble of operating the system independently and able to build their own sensrors. This year saw a complete overhaul of her system including the installation of updated software.