MYOCELLULAR ENERGY METABOLISM: STUDIES USING SELF-REFERENCING MICROELECTRODES

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定是研究者机构。哈珀博士的研究重点是肌肉葡萄糖转运蛋白的分子生理学，因为它与II型糖尿病有关。该组织内的葡萄糖隔离不足被认为是疾病进展的一个明显并发症。 测量葡萄糖转运以前是复杂和缓慢的。然而，由BioCurrents研究中心开发的自参考葡萄糖微传感器的出现大大提高了我们在单细胞水平上检查运输动力学的能力-这是哈珀博士实验室研究的主题。 为了方便这项研究，哈珀博士在渥太华的实验室安装了一套完整的电流分析系统。该小组现在完全能够独立操作系统，并能够建立自己的传感器。 今年，她的系统进行了全面检修，包括安装了更新的软件。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Dr Harper's studies focus on the molecular physiology of the muscle glucose transporter as it relates to Type II diabetes. Insufficient glucose sequestration within this tissues is accepted as one clear complication of the disease progression. Measuring glucose transport has previously been complicated and slow. However, the advent of the self-referencing glucose microsensor, developed by the BioCurrents Research Center, has greatly improved our ability to examine the dynamics of transport at the single cell level - the subject of the studies in Dr Harper's laboratory. To facilitate this research a full amperometric system was installed in Dr Harper's laboratory in Ottawa. The group is now fully capapble of operating the system independently and able to build their own sensrors. This year saw a complete overhaul of her system including the installation of updated software.