COPPER AND IRON HOMEOSTASIS IN YEAST

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定是研究者机构。铜和铁是所有好氧生物体所需的两种必需营养素，用于许多生物过程，包括能量产生，保护免受氧化应激和氧运输等。从酵母到哺乳动物，铜和铁稳态的机制是高度保守的，并且关于后生动物中这些营养素的摄取和分布机制的许多已知都来自酵母的研究。我们实验室的一个研究领域旨在了解酵母细胞如何通过这种金属从细胞外环境穿过质膜的协调运输获得铜，以及铜穿过细胞内膜的动员。来自我们实验室和其他实验室的证据表明，特定的金属还原酶是酵母中高亲和力铜和铁摄取所必需的，但这些金属还原酶与转运蛋白的功能和潜在物理相互作用的细节目前尚不清楚。在这里，我们建议调查的质膜金属还原酶Fre 1和铜转运蛋白Ctr 1在酵母中使用FRET之间的潜在物理相互作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Copper and iron are two essential nutrients required by all aerobic organisms for numerous biological processes including energy generation, protection from oxidative stress, and oxygen transport, among others. The mechanisms of copper and iron homeostasis are highly conserved from yeast to mammals, and much of what is known about the mechanisms of uptake and distribution of these nutrients in metazoans has come from studies in yeast. One area of investigation in our lab is aimed at understanding how yeast cells acquire copper through the coordinated transport of this metal from the extracellular environment across the plasma membrane, as well as the mobilization of copper across intracellular membranes. Evidence from our lab and others suggests that specific metalloreductases are required for high-affinity copper and iron uptake in yeast, but the details of the functional and potential physical interaction of these metalloreductases with the transport proteins are currently not understood. Here we propose to investigate the potential physical interaction between the plasma membrane metalloreductase Fre1 and the copper transporter Ctr1 in yeast using FRET.