SCHIMMEL PRT-CRYSTAL STRUCTURE OF TRBP111/TRNA COMPLEX

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。Trbp111是一个古老的、结构特异性的tRNA识别结构域，存在于许多新的、人类凋亡相关的细胞因子、氨基酰基tRNA合成酶和其他蛋白质合成装置的组成部分中。该结构域的功能是识别tRNA的一般l形状，无论其序列如何，并将其传递给其受体酶进行有效的氨基酰化。我想在原子水平上理解这种结构特异性tRNA识别的本质。因此，我测定了细菌Trbp111的晶体结构，发现了一个具有两个OB-fold结构域的同型二聚体和一个新的二聚体界面（Swairjo et al., EMBO J, 19: 6287,2000）。我现在正在研究tRNA复合物的晶体结构。我已经在内部设备上生产了Aquifex aeolicus Trbp111与大肠杆菌tRNAfMet络合的晶体，其衍射值为7.0¿¿。我希望在SSRL收集更高分辨率的x射线数据，以便确定复杂的结构。这个项目是我们研究最近在基因组数据库中发现的几个新的、非特异性的RNA识别域的总体兴趣的一部分（Avarind和Koonin， J. Mol. Evol）。科学通报，38:291,1999)。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Trbp111 is an ancient, structure-specific tRNA recognition domain found in many novel, human apoptosis-related cytokines, aminoacyl-tRNA synthetases, and other components of the protein synthesis apparatus. The function of this domain is to recognize the general L-shape of tRNA, regardless of sequence, and deliver it to its receptor enzyme for efficient aminoacylation. I want to understand the nature of this structure-specific tRNA recognition at an atomic level. Therefore, I determined the crystal structure of bacterial Trbp111 which revealed a homodimer with two OB-fold domains and a novel dimer interface (Swairjo et al., EMBO J, 19: 6287, 2000). I am now pursuing the crystal structure of the complex with tRNA. I have produced crystals of Aquifex aeolicus Trbp111 complexed with E. coli tRNAfMet which diffract to 7.0 ¿¿on in-house equipment. I hope to collect higher-resolution X-ray data at SSRL that would allow for determination of the complex structure. This project is part of our general interest in studying several, novel, non-specific RNA recognition domains identified recently in genomic databases (Avarind and Koonin, J. Mol. Evol., 38: 291, 1999).