KINETICS AND THERMODYAMIC RNA FOLDING INTERMEDIATES MONITORED BY SAXS
SAXS 监测的动力学和热力 RNA 折叠中间体
基本信息
- 批准号:7370440
- 负责人:
- 金额:$ 0.13万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2006
- 资助国家:美国
- 起止时间:2006-03-01 至 2007-02-28
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. RNA, once thought to serve mainly as a courier of genetic information, is now known to play central structural and enzymatic roles in such complexes as the spliceosome and ribosome. To function, RNA must fold to a discrete three-dimensional structure, which introduces the RNA folding problem. Our previous SAXS work demonstrated that the Tetrahymena group I ribozyme undergoes a large, Mg2+-dependent collapse in folding from the denatured state to the folded state, and that collapse occurs substantially faster than overall folding. Further, we found that collapse is largely complete before any tertiary structure is observed to form, suggesting that a non-specifically collapsed state is populated early in folding. Here we propose to build on these observations by (i) obtaining structural information on the progression of intermediates that is populated after formation of the collapsed state; (ii) probing molecular features that affect the properties of the collapsed state; and (iii) monitoring the global structures of intermediates that are populated as the ribozyme is denatured by urea. These experiments are part of a larger project aimed at characterizing the folding behavior and the overall folding landscape for this RNA.
该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者(PI)可能从另一个NIH来源获得主要资金,因此可以在其他CRISP条目中表示。所列机构为中心,不一定是研究者所在机构。RNA,曾经被认为主要是作为遗传信息的信使,现在已知在剪接体和核糖体等复合体中起着重要的结构和酶作用。为了发挥功能,RNA必须折叠成离散的三维结构,这就引入了RNA折叠问题。我们以前的SAXS工作表明,四膜虫组I核酶经历了一个大的,Mg 2+依赖的折叠折叠从变性状态到折叠状态的崩溃,崩溃发生的速度大大快于整体折叠。此外,我们发现,崩溃基本上是在观察到任何三级结构形成之前完成的,这表明一个非特异性的崩溃状态在折叠早期被填充。在这里,我们建议建立在这些观察(i)获得结构信息的进展后,填充的崩溃状态形成的中间体;(ii)探测分子特征,影响的崩溃状态的属性;和(iii)监测的全局结构的中间体填充的核酶是由尿素变性。这些实验是一个更大的项目的一部分,旨在表征这种RNA的折叠行为和整体折叠景观。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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DANIEL HERSCHLAG其他文献
DANIEL HERSCHLAG的其他文献
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{{ truncateString('DANIEL HERSCHLAG', 18)}}的其他基金
INCISIVE PROBING OF NUCLEIC ACID CONFORMATIONAL HETEROGENEITY
深入探究核酸构象异质性
- 批准号:
8362312 - 财政年份:2011
- 资助金额:
$ 0.13万 - 项目类别:
INCISIVE PROBING OF NUCLEIC ACID CONFORMATIONAL HETEROGENEITY
深入探究核酸构象异质性
- 批准号:
8170316 - 财政年份:2010
- 资助金额:
$ 0.13万 - 项目类别:
STRUCTURAL INFERENCE OF NATIVE AND PARTIALLY FOLDED RNA BY CONTACT MAPPING
通过接触图谱对天然和部分折叠 RNA 进行结构推断
- 批准号:
7957682 - 财政年份:2009
- 资助金额:
$ 0.13万 - 项目类别:
KINETICS AND THERMODYAMIC RNA FOLDING INTERMEDIATES MONITORED BY SAXS
SAXS 监测的动力学和热力 RNA 折叠中间体
- 批准号:
7180420 - 财政年份:2005
- 资助金额:
$ 0.13万 - 项目类别:
KINETICS & THERMODYAMIC RNA FOLDING INTERMEDIATES MONITORED BY SAXS
动力学
- 批准号:
6976329 - 财政年份:2004
- 资助金额:
$ 0.13万 - 项目类别:
Project 2 Enzymatic activity and structure probing
项目2 酶活性和结构探测
- 批准号:
6760473 - 财政年份:2003
- 资助金额:
$ 0.13万 - 项目类别:
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