THE STRUCTURE OF SOXAX: A CYTOCHROME WITH A ROLE IN THIOSULFATE OXIDATION

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。还原的含硫化合物可作为电子供体被多种微生物利用，支持自养生长。硫代硫酸盐是自然界中还原硫最丰富的形式之一，许多微生物都有氧化这种化合物的能力。研究最多的硫代硫酸盐氧化途径涉及硫代硫酸盐氧化多酶体系(TOMS)，该体系可以将硫代硫酸盐、亚硫酸盐或硫化物氧化成硫酸盐，并以细胞色素c为电子受体。该途径由硫氧化(Sox)基因簇编码，含有血红素蛋白(SoxB、SoxC)和多种细胞色素(SoxA、Soxx、SoxD、SoxE)。SoxAX是一种异二聚体蛋白，含有约30 kDa和15 kDa的两个含血红素c的亚基。这里要研究的SoxAX是来自Starkeya Novell的SoxAX

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Reduced sulfur compounds can be used a electron donors by a variety of microorganisms, supporting autotrophic growth. Thiosulfate is one of the most abundant forms of reduced sulfur in Nature, and the ability to oxidise this compound is found in many microorganisms. The best studied pathway of thiosulfate oxidation involves the thiosulfate-oxidising multi-enzyme system (TOMES) which can oxidise thiosulfate, sulfite or sulfide to sulfate with cytochrome c as the electron acceptor. This pathway is encoded by sulfur oxidation (sox) gene clusters and contains heme proteins (SoxB, SoxC) as well as a number of cytochromes (SoxA, SoxX, SoxD, SoxE). SoxAX is heterodimeric protein with two heme c-bearing subunits of approximately 30 and 15 kDa, respectively. The SoxAX to be studied here is that from Starkeya novell