INTESTINAL ENDOGENOUS ZINC: ROLE FOR METALLOTHIONEIN

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。锌缺乏症已在全球范围内得到充分证明，缺锌对健康的不利影响也是如此。锌在人体内有许多重要的功能，但关于锌的确切用途仍有许多需要了解。健康的人通过调节食物对锌的吸收进入肠道，并控制锌的释放回到肠道以排出粪便，从而平衡体内的锌含量。大多数研究都集中在肠道对锌的吸收上。这项研究将更好地描述锌向肠道分泌的生理和影响因素。更好地了解人体通常如何达到适当的锌平衡，可能有助于最终治疗和/或预防缺锌。这项研究将调查一种名为金属硫蛋白的蛋白质是否控制锌向肠道的分泌。根据动物研究的实验数据和我们自己的初步数据，我们假设从胰腺到小肠的分泌物中金属硫蛋白的量与这些分泌物中的锌的量直接相关。这项研究还将评估胰腺分泌物中金属硫蛋白的数量是否会随着从食物中吸收的锌量的变化以及体内锌负荷的变化而变化。20-50岁的健康成年人将从丹佛大都会地区招募。我们将对10名受试者进行研究，他们的饮食中的锌水平与他们平时的饮食水平相似。然后，受试者将被随机分配到限制或补充锌的饮食中。在他们开始他们指定的饮食之前和之后，受试者将在GCRC中度过2天，在那里将一根小管插入每个受试者的口腔，并沿着他/她的喉咙向下延伸到他/她的肠道。这个管子将被用来吸出分泌物，然后我们将对其进行分析。所有受试者还将在研究的不同日子接受一些特殊形式的锌。这些将有助于我们测量分泌物收集物中的锌含量

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Zinc (Zn) deficiency has been amply demonstrated on a global basis, as have the adverse implications of Zn deficiency on health. Zn has many important functions in the body, and yet much remains to be learned about exactly how Zn is used. Healthy individuals balance the amount of Zn in their bodies by regulating absorption of Zn from food into the gut and by controlling the release of zinc back into the intestine for excretion in feces. Most research has focused on zinc absorption by the gut. This study will better characterize the physiology of and factors affecting zinc secretion into the intestine. An improved understanding of the ways in which the body usually achieves proper zinc balance may help to ultimately treat and/or prevent Zn deficiency. This study will investigate if a protein, metallothionein, controls Zn secretion into the intestine. Based on experimental data from animal studies and our own preliminary data, we hypothesize that the amount of metallothionein in secretions from the pancreas into the small intestine is directly related to the amount of Zn in these secretions. This study will also evaluate whether the amount of metallothionein in secretions from the pancreas changes when the amount of zinc absorbed from food changes and when the Zn load in the body changes. Healthy adults aged 20-50 will be recruited from the Denver metro area. We will study 10 subjects on a diet with Zn levels similar to their usual diets. Subjects will then be randomly assigned to a Zn restricted or supplemented diet. Before and after they begin their assigned diet, subjects will spend 2 days in the GCRC, where a small tube will be inserted into each subject's mouth and advanced down his/her throat to his/her intestine. This tube will be used to suck out secretions that we will then analyze. All subjects will also receive some special forms of Zn on different days in the study. These will help us to measure Zn amounts in the secretions collecte