EFFECT OF MCPA 2,4 D AND BROMOXYNIL ON LUNG DEVELOPMENT

MCPA 2,4 D 和溴苯腈对肺发育的影响

• 批准号: 7381582
• 负责人: HILDE E VAN GIJSSEL
• 金额: $ 18.23万
• 依托单位: UNIVERSITY OF NORTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7381582
• 关键词: EFFECT; MCPA; BROMOXYNIL; LUNG; DEVELOPMENT

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Results of studies proposed will increase our understanding about the toxic effects chlorophenoxy herbicides and will provide insight in the use of Drosophila for studies on human health. Most of the past year was occupied with buying equipment and supplies and building a workable laboratory space. Experiments were begun in January 2005. Our collaborator Mary Stewart at NDSU showed us the basics for Drosophila culture and techniques used for exposure of flies and embryos to herbicides, and harvesting flies and embryos. For Specific Aim 1 we will develop a model to study tracheal development of the Drosophila. Experiment 1 will establish the LC 50 of 2,4-D, MCPA and Bromoxynil. The first experiments done with 2,4-D were not successful because all the flies died due to the ethanol used as a solvent. Experiments using a different solvent (3% ethanol, 1 % Tween 80 in water) are currently underway. Also alternative exposure on filter papers is being tested. LC 50 experiments for MCPA and Bromoxynil are underway. For Experiment 2 we study the effect of the model compounds on the life cycle of the flies. Currently experiments are being done to establish a detailed life cycle in our laboratory for normal flies. An incubator that maintains humidity and temperature and day and night cycle is used in these experiments. For Specific aim 2 we have started experiments to optimize mRNA and protein extraction from Drosophila embryos and of culturing large quantities of Drosophilas. The goals for the next funding period are to finish the experiments of Specific Aim 1 and start the experiments of Aim 2. Currently students are recruited for the research in the summer of 2005. Connections with the tribal college have been made with the goal to easy transition of Native American students from a 2-year college to a 4-year college and increase the participation of Native Americans in biomedical research.

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。提出的研究结果将增加我们对氯苯氧基除草剂的毒性作用的了解，并将为利用果蝇进行人类健康研究提供洞察力。过去一年的大部分时间都在购买设备和用品，并建造一个可行的实验室空间。实验于2005年1月开始。我们在南加州州立大学的合作者玛丽·斯图尔特向我们展示了果蝇培养的基础知识，以及将果蝇和胚胎暴露在除草剂中，以及收获果蝇和胚胎所使用的技术。针对特定目的1，我们将开发一种模型来研究果蝇的气管发育。实验1将确定2，4-D、MCPA和溴苯腈的半数致死浓度。最初用2，4-D进行的实验并不成功，因为所有的苍蝇都死于乙醇作为溶剂。使用不同溶剂(3%乙醇，1%吐温80水)的实验目前正在进行中。此外，还在测试滤纸上的替代暴露。MCPA和Bromoxynil的LC 50试验正在进行中。在实验2中，我们研究了模型化合物对苍蝇生活史的影响。目前，我们实验室正在进行实验，以建立正常果蝇的详细生命周期。在这些实验中，使用了一个保持湿度和温度以及昼夜循环的孵化器。针对特定的目标2，我们已经开始了从果蝇胚胎中优化mRNA和蛋白质提取以及大量培养果蝇的实验。下一个资助期的目标是完成具体目标1的实验，并开始目标2的实验。目前正在招募学生参加2005年夏天的研究。与部落学院建立了联系，目的是使美洲原住民学生从两年制大学轻松过渡到四年制大学，并增加美洲原住民对生物医学研究的参与。