CORTICOTROPIN RELEASING FACTOR IN THE CENTRAL NUCLEUS OF THE AMYGDALA IN STRESS

应激状态下杏仁核中央核的促肾上腺皮质激素释放因子

• 批准号: 7381713
• 负责人: PATRICK J RONAN
• 金额: $ 0.22万
• 依托单位: UNIVERSITY OF SOUTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7381713
• 关键词: CORTICOTROPIN; RELEASING; FACTOR; CENTRAL; NUCLEUS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Stress-induced psychopathologies are major problems affecting society. Disorders ranging from post-traumatic stress disorder (PTSD), depression and sleep dysregulation to lapse and relapse in addiction all have stress as an etiological factor. The mechanisms underlying these stress-induced disorders are poorly understood. Corticotropin releasing factor (CRF) is a molecule in brain that has been implicated as a primary coordinator of an organism?s adaptive stress responses - including endocrine, autonomic and behavioral responses. Numerous brain regions have been implicated in CRF mediated stress responses. The central nucleus of the amygdala (CeA) is a major extrahypothalamic source of CRF and is known to mediate behavioral, emotional and physiological responses to fear and anxiety. The goals of this project are to elucidate the specific role of corticotropin releasing factor (CRF) from the CeA in central responses to stress. Students will be involved in specific experiments in which we will: 1. Determine if CRF from the CeA is involved in mediating stress-induced and anxiety-like behaviors. RNA interference (RNAi) will be used to locally silence CRF expression bilaterally in the CeA of adult rats. The rats will then be put through a battery of behavioral paradigms to measure motor and anxiety-like behaviors. These tests include the open field test, elevated plus maze, light-dark box, and acoustic startle. 2. Determine if knocking down CRF expression from the CeA using RNAi decreases measures of neuroendocrine and autonomic activation in response to stress. Heart rate and blood pressure will be monitored employing radiotelemetry. Circulating levels of corticosterone and adrenocorticotropic hormone (ACTH) will also be measured

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。压力引起的精神病理是影响社会的主要问题。从创伤后应激障碍（PTSD）、抑郁、睡眠失调到成瘾的消失和复发等疾病都有压力作为病因。这些应激性疾病的机制尚不清楚。促肾上腺皮质激素释放因子（CRF）是大脑中的一种分子，被认为是生物体的主要协调者。适应性应激反应——包括内分泌、自主神经和行为反应。许多大脑区域都与CRF介导的应激反应有关。杏仁核中央核（CeA）是CRF的主要下丘脑外来源，并介导对恐惧和焦虑的行为、情绪和生理反应。本项目的目的是阐明来自CeA的促肾上腺皮质激素释放因子（CRF）在中枢应激反应中的具体作用。学生将参与具体的实验，我们将：1。确定来自CeA的CRF是否参与介导应激诱导和焦虑样行为。RNA干扰（RNAi）将用于局部沉默成年大鼠CeA中CRF的表达。然后，这些老鼠将经历一系列的行为范式，以测量运动和焦虑类行为。这些试验包括野外试验、高架加迷宫试验、明暗箱试验和声惊吓试验。2. 确定使用RNAi降低CeA的CRF表达是否会降低应激反应中的神经内分泌和自主神经激活的测量。心率和血压将由无线电遥测技术监测。循环水平的皮质酮和促肾上腺皮质激素（ACTH）也将被测量