Stem Cell Derivation Of Two Neotropical Primate Species

简介（由申请人提供）：本计划为支持南阿拉巴马大学比较医学系助理教授a. Michele Schuler博士的科学发展而设立。她于1993年在宾夕法尼亚州立大学（Penn State University）获得动物生物科学学士学位，1997年在奥本大学（Auburn University）获得医学博士学位，并获得aclam认可的博士后奖学金/博士学位。2003年在阿拉巴马大学伯明翰分校攻读分子和细胞病理学专业。该项目的总体目标是优化新热带灵长类动物的辅助生殖技术，最终目标是从这些物种中开发胚胎干细胞系。舒勒博士召集了一组知名的研究人员（南阿拉巴马大学的Jonathan Scammell博士和Botros Rizk博士；德克萨斯州巴斯特罗普MD安德森癌症中心的Christian R. Abee博士；俄勒冈州国家灵长类动物研究中心的Donald Wolf博士和Mary Zelinski- Wooten博士；德克萨斯州坦普尔斯科特怀特医院的Thomas Kuehl博士；佐治亚州亚特兰大埃默里大学的Anthony Chan博士），他们为申请人制定了详细的培训计划。该项目的具体目标是：(1)确定松鼠猴和猫头鹰猴卵巢刺激和卵母细胞收集的最佳策略；(2)确定松鼠和猫头鹰猴细胞质内精子注射（ICSI）和/或体外受精（IVF）的最佳方法，包括卵母细胞和胚胎的最佳培养技术；(3)优化子宫冲洗法提取体外胚泡的条件；(4)从松鼠猴和猫头鹰猴身上培育胚胎干细胞系。该应用程序利用了美国新热带灵长类动物研究和资源中心（CNPRR）的独特环境，并响应了国家研究资源中心2004-2008年战略计划中概述的项目目标。

DESCRIPTION (provided by applicant): This is a SERCA to support the scientific development of Dr. A. Michele Schuler, an Assistant Professor in the Department of Comparative Medicine at the University of South Alabama. Dr. Schuler received her B.S. in Animal Bioscience at Penn State University in 1993, her D.V.M. from Auburn University in 1997, and her Ph.D. from the ACLAM-accredited postdoctoral fellowship/Ph.D. program in Molecular and Cellular Pathology at the University of Alabama at Birmingham in 2003. The overall goal of the project described in this application is to optimize assisted reproductive technologies in neotropical primates with the ultimate goal of developing embryonic stem cell lines from these species. Dr. Schuler has assembled a panel of well established investigators (Dr. Jonathan Scammell and Dr. Botros Rizk at the University of South Alabama; Dr. Christian R. Abee at MD Anderson Cancer Center, Bastrop, TX; Dr. Donald Wolf and Dr. Mary Zelinski- Wooten at Oregon National Primate Research Center; Dr. Thomas Kuehl at Scott and White Hospital, Temple, TX; and Dr. Anthony Chan at Emory University, Atlanta, GA) who have developed a detailed training program for the applicant. The specific aims of the project are: (1) To determine the best strategy for ovarian stimulation and oocyte collection in squirrel monkeys and owl monkeys; (2) To determine the optimal method for intra-cytoplasmic sperm injection (ICSI) and/or in vitro fertilization (IVF) in squirrel and owl monkeys including optimal culture techniques for oocytes and embryos; (3) To optimize conditions for retrieval of in vivo-derived blastocysts by uterine flushing; (4) To develop embryonic stem cell lines from squirrel monkeys and owl monkeys. This application takes advantage of the unique environment of the Center for Neotropical Primate Research and Resources (CNPRR) at USA and is responsive to programmatic objectives outlined in the National Center for Research Resources 2004-2008 Strategic Plan.