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Cytoplasmic polyadenylation in NIH3T3 cells

NIH3T3 细胞中的细胞质多聚腺苷酸化

基本信息

批准号：
BB/G001847/1
负责人：
Cornelia Huiberdina De Moor
金额：
$ 67.36万
依托单位：
University of Nottingham
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2008
资助国家：
英国
起止时间：
2008 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FG001847%2F1
关键词：
Cytoplasmic polyadenylation NIH3T3 cells

项目摘要

Regulation of gene expression determines the level of specific proteins in a cell and can be controlled in many ways to allow processes such as growth and the prevention of disease. Cytoplasmic polyadenylation is a form of this regulation by which the production of protein from an mRNA can be controlled. It is very important in eggs and has also been detected in the brain. However it is not clear whether it plays a role in any other types of cells. We have recently shown that cytoplasmic polyadenylation also occurs when fibroblasts attach to a new surface. Fibroblasts are skin cells and their attachement contributes to skin healing. Fibroblasts are much less specialised than eggs or brain cells, indicating that regulation by cytoplasmic polyadenylation may be a general phenomenon. We are now planning to investigate how widespread cytoplasmic polyadenylation is in fibroblasts and what proteins are required for this process. The data gathered in this project will ultimately have relevance for many medical problems, such as wound healing and the spreading of cancer cells through the body.

基因表达的调节决定了细胞中特定蛋白质的水平，并且可以通过许多方式进行控制，以允许诸如生长和预防疾病等过程。胞质多聚腺苷酸化是这种调节的一种形式，通过这种调节可以控制mRNA产生蛋白质。它在鸡蛋中非常重要，在大脑中也被检测到。但目前尚不清楚它是否在其他类型的细胞中发挥作用。我们最近发现，当成纤维细胞附着在新的表面上时，细胞质多聚腺苷酸化也会发生。成纤维细胞是皮肤细胞，它们的附着有助于皮肤愈合。成纤维细胞比卵细胞或脑细胞的特化程度低得多，这表明细胞质聚腺苷酸化的调节可能是一种普遍现象。我们现在正计划研究细胞质多聚腺苷酸化在成纤维细胞中有多普遍，以及这一过程需要哪些蛋白质。该项目收集的数据最终将与许多医学问题相关，例如伤口愈合和癌细胞在体内的扩散。

项目成果

期刊论文数量（8）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Nuclear poly(A) tail size is regulated by Cnot1 during the serum response

血清反应期间核聚腺苷酸尾部大小受 Cnot1 调节

DOI：
10.1101/773432
发表时间：
2019
期刊：
影响因子：
0
作者：
Singhania R
通讯作者：
Singhania R

Structural insights into the targeting of mRNA GU-rich elements by the three RRMs of CELF1.

DOI：
10.1093/nar/gkt470
发表时间：
2013-08
期刊：
Nucleic acids research
影响因子：
14.9
作者：
Edwards JM;Long J;de Moor CH;Emsley J;Searle MS
通讯作者：
Searle MS

Genome-Wide Analysis of PAPS1-Dependent Polyadenylation Identifies Novel Roles for Functionally Specialized Poly(A) Polymerases in Arabidopsis thaliana.

PAPS1依赖性聚腺苷酸化的全基因组分析确定了拟南芥功能专业的聚（A）聚合酶的新作用。

DOI：
10.1371/journal.pgen.1005474
发表时间：
2015-08
期刊：
PLoS genetics
影响因子：
4.5
作者：
Kappel C;Trost G;Czesnick H;Ramming A;Kolbe B;Vi SL;Bispo C;Becker JD;de Moor C;Lenhard M
通讯作者：
Lenhard M

Sequence determinants for the tandem recognition of UGU and CUG rich RNA elements by the two N--terminal RRMs of CELF1.