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Animal Studies

动物研究

基本信息

批准号：
7728853
负责人：
ROSALYN D BLUMENTHAL
金额：
$ 19.51万
依托单位：
CTR FOR MOLECULAR MEDICINE/IMMUNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-08-01 至 2009-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7728853
关键词：
Animals Antibodies Antigens Apoptotic Biodistribution Biological Assay Bispecific Antibodies Blood Blood Platelets Blood specimen Body Weight Body Weight Changes Cardiac Cell Line Cells Clinical Chemistry Collection Combined Modality Therapy Cultured Tumor Cells Data Development Dose Excision Formalin Haptens Hematopoietic Hemorrhage Histology Image Individual Injection of therapeutic agent Laboratories Laboratory Animals Leukocytes Lymphoma Measurement Measures Modeling Monitor Multiple Myeloma Mus Nude Mice Paraplegia Peripheral Platelet Count measurement Proteins Protocols documentation Radioimmunoconjugate Radioimmunodetection Radiolabeled Route SCID Mice Sampling Services Therapeutic Agents Tissue Sample Tissues Toxic effect Treatment Efficacy Troponin Tumor Tissue Veins antibody conjugate chemotherapy immunosuppressed implantation microautoradiography pre-clinical radiotracer response size subcutaneous tumor tumor initiation tumor xenograft uptake

项目摘要

Core C is the animal core. The core will provide technical service to preclinical projects 1, and 2 establishing subcutaneous and systemic tumor models, assessing uptake of radiolabeled agents, determining therapeutic efficacy of radiolabeled antibody conjugates and haptens, and identifying toxicity associated with single and multimodal treatment protocols. It will also collect blood for toxicity assessments and tissue for the histological analysis .by Core E. It will use 2 F/T technicians to manage 2160-2560 mice per year in its AAALAC-accredited facility. Specifically it will be responsible for the following tasks: (a) developing new tumor models in untreated and immunosuppressed mice, (b) injecting cultured tumor cells intravenously or subcutaneously in SCID mice or nude mice, (c) dosing animals with appropriate therapeutic agents [e.g., bispecific antibodies, radiolabeled haptens, radioantibodies, naked antibodies, and chemotherapeutics], (d) monitoring survival, body weight changes and onset of hind-leg paralysis in systemic tumor models and measuring tumor size of subcutaneous tumors by 3D caliper measurements, (e) performing biodistribution studies, (f) providing fixed samples of tumors and tissues for histology to monitor viability and to monitor expression of antigens by immunohistology and for microautoradiography studies, (g) collecting blood samples for white blood cell and platelet enumeration and for measurement of cardiac troponin in project 4, (h) entering all data (biodistribution, therapy, WBC/Plt, body weight) into Excel spreadsheets, and (i) setting up immunoscintigraphy studies.

核心C是动物核心。核心将为临床前项目1、2的建立提供技术服务