Retroviral Transduction Core

该计划中项目的许多实验方案依赖于逆转录病毒转导初级 或培养的细胞。因为逆转录病毒的制备和转导技术需要高度的 技术专长，一个执行这些技术的中央设施将显著提高 所有项目的进展情况。用于逆转录病毒制备和转导的中心设施将提供多种 集团的优势所在。这些措施包括：1)制备高滴度逆转录病毒的统一程序 上清液，2)高滴度逆转录病毒上清液的定期供应，3)逆转录病毒的定期监测 上清液以确保它们不含支原体并且不含有复制能力强的逆转录病毒， 以及4)胎肝和骨髓造血干细胞转导方面的专业知识，因为 研究计划的协作性、高滴度逆转录病毒上清液的可用性和程序 对于转导，与标准化的方案和试剂将是非常有益的 项目中的每一个人。逆转录病毒核心将被安置在Pear博士的研究空间中。核心意志 购买和维护这些程序所需的设备。此外，消耗品也将被 核心批量采购，以确保材料标准化和批量采购折扣。

Many of the experimental protocols for the projects in this program rely on retroviral transduction of primary or cultured cells. Because the techniques of retroviral preparation and transduction require a high degree of technical expertise, a central facility where these techniques are performed would significantly enhance the progress of all projects. A central facility for retroviral preparation and transduction would offer multiple advantages to the group. These include: 1) uniform procedures for preparing high titer retroviral supernatants, 2) regular availability of high titer retroviral supernatants, 3) periodic monitoring of retroviral supernatants to insure that they are mycoplasma-free and do not contain replication competent retrovirus, and 4) expertise in transduction of fetal liver and bone marrow hematopoietic stem cells, Because of the collaborative nature of the research program, availability of high titer retroviral supernatants and procedures for transduction, together with standardization of protocols and reagents would be of great benefit to everyone in the program. The retroviral core will be housed in Dr. Pear's research space. The Core will purchase and maintain equipment necessary for these procedures. Additionally, consumables will also be purchased in bulk by the Core to ensure standardization of materials and bulk purchase discounts.