Suppressor CD8+ T cells in HIV infection

HIV 感染中的抑制性 CD8 T 细胞

• 批准号: 7624159
• 负责人: Mohamed M Elrefaei
• 金额: $ 5.67万
• 依托单位: CA ST DEPT OF HLTH SRVS--DIV OF LAB SCI
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-05 至 2010-06-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7624159
• 关键词: Appendix; CD8B1 gene; Cells; Characteristics; Cytolysis; Disease; HIV; HIV Infections; IL2RA gene; Immune System Diseases; Immune response; In Vitro; Infection; Interleukin-10; Mediating; Plasma; Population; RNA; Research; Role; Series; Specificity; Suppressor-Effector T-Lymphocytes; T-Lymphocyte; Transforming Growth Factor beta; cytokine; design; in vivo; research study; response

DESCRIPTION (provided by applicant): CD8+ T suppressor lymphocytes have been described. However, the regulatory role of IL-10+ CD8+ Ts in modulating HIV-specific T cell responses remains unknown. The aims proposed in this research plan contain a series of experiments designed to explore the role of HIV-specific IL-10+ CD8+ T cells in mediating T cell immune dysfunction in HIV infection. I hypothesize that HIV-specific IL-10+ CD8+ T cells exert an inhibitory effect on distinct populations of HIV-specific effector T cells. Aim 1 explores the mechanisms of suppression utilized by the IL-10+ CD8+ Ts during HIV infection. The role of 1) contact dependent suppression, 2) cytokines such as IL-10 and TGF-beta, will be examined. Aim 2 investigates the phenotypic characteristics and specificity of the IL-10+ CD8+ Ts during HIV infection. This aim will focus on examining the suppressive effect of the IL-10+ CD8+ Ts to different HIV, and non-HIV-specific effector T cells. The expression by the IL- 10+ CD8+ Ts of various markers associated with regulatory T cells, such as CD25, FoxpS, CTLA-4 will also be assessed.

描述（由申请人提供）：CD8+ T抑制淋巴细胞已被描述。然而，IL-10+ CD8+ T在调节hiv特异性T细胞反应中的调节作用尚不清楚。本研究计划提出的目标包括一系列旨在探索HIV特异性IL-10+ CD8+ T细胞在介导HIV感染中T细胞免疫功能障碍中的作用的实验。我假设hiv特异性IL-10+ CD8+ T细胞对不同群体的hiv特异性效应T细胞有抑制作用。目的1探讨IL-10+ CD8+ t在HIV感染期间的抑制机制。1)接触依赖性抑制，2)细胞因子如IL-10和tgf - β的作用将被检查。目的2研究HIV感染期间IL-10+ CD8+ t的表型特征和特异性。这一目标将集中于检测IL-10+ CD8+ T对不同HIV和非HIV特异性效应T细胞的抑制作用。还将评估IL- 10+ CD8+ T细胞中与调节性T细胞相关的各种标志物（如CD25、FoxpS、CTLA-4）的表达。