Biomechanics and Imaging Core

生物力学和成像核心

• 批准号: 7347203
• 负责人: THOMAS DUDLEY BROWN
• 金额: $ 42.31万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-10 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7347203
• 关键词: Animal Model; Animals; Biomechanics; Cartilage; Cartilage injury; Contusions; Custom; Degenerative polyarthritis; Device Designs; Disease Progression; Elevation; Experimental Models; Family suidae; Fracture; Future; Human; Image; Intervention; Iowa; Joint Instability; Knee; Longitudinal Studies; Measurement; Modeling; Monitor; Operative Surgical Procedures; Organ; Oryctolagus cuniculus; Patients; Pharmacologic Substance; Physiologic pulse; Preparation; Process; Pulse taking; Rate; Sampling; Secondary to; Serum; Stress; Therapeutic Effect; Time; Tissue Banks; Tissues; Universities; absorption; bone; cost; genetic risk factor; tissue preparation; transmission process

A Tissue & Experimental Modeling Core will coordinate specialized tissue preparations and animal models, and will serve Projects 1, 2, and 3 of the University of Iowa CORT. All animal modeling studies will be managed by this Core, to maximize the efficiency in time, cost, and effort. This Core will provide two survival animal models: a porcine knee model of blunt impaction cartilage injury (the associated studies are proposed in Projects 1, 2, and 3), and a rabbit knee model of blunt impaction with joint instability (for Project 2). Surgical insults for these models will be applied arthroscopically, to minimize potential effects of surgical invasion itself. Impaction insults will be performed using a custom-designed device that allows delivery of a compressive force pulse of prescribed energy magnitude. The force transmission during the impaction will be monitored, allowing accurate measurement of energy absorption, the peak cartilage stress level, and the rate of cartilage stress elevation. All tissue collection from experimental animals, including cartilage and serum samples, and the processing and storing of these samples, will also be managed by this Core. Cartilage sample preparations for ex vivo studies in Projects 1, 2, and 3, namely the modeling of blunt impaction cartilage damage associated with intraarticular fractures or bone bruises, will also be provided by this Core. Another objective of this Core is to facilitate preparation for future studies. To this end, this Core aims to pilot an animal model of human intraarticular fracture that naturally develops post-traumatic osteoarthritis (PTOA). This model will allow not only longitudinal study of the disease progression processes of PTOA secondary to intraarticular fractures, but will also allow future studies of the organ-level therapeutic effects of new treatment paradigms, including bio-pharmaceutical interventions that will be studied in Projects 1 and 2. A porcine model of knee instability will also be piloted. In addition to these tasks, this Core will collect and store serum samples from all patients in Projects 3 and 4, to enable future studies of possible genetic risk factors for post-traumatic OA in humans.

组织和实验建模核心将协调专门的组织制剂和动物模型， 并将为爱荷华大学科特大学的项目1、2和3提供服务​​。所有动物建模研究将是 由该核心管理，以最大程度地提高时间，成本和精力的效率。这个核心将提供两个生存 动物模型：钝性撞击软骨损伤的猪膝关节模型（提出了相关的研究 在项目1、2和3）中，以及与关节不稳定性的兔子膝盖模型（针对项目2）。 这些模型的手术侮辱将通过关节镜应用，以最大程度地减少手术的潜在影响 入侵本身。 Impaction侮辱将使用定制设计的设备进行，该设备允许交付 规定能量幅度的压缩力脉冲。撞击期间的力传输将是 受到监测，可以准确测量能量吸收，峰值软骨应力水平和速率 软骨应力升高。来自实验动物的所有组织收集，包括软骨和血清 样本以及这些样品的处理和存储也将由该核心管理。软骨 在项目1、2和3中进行过体内研究的样本制备，即钝性撞击的建模 该核心也将提供与关节内裂缝或骨瘀伤相关的软骨损伤。 该核心的另一个目的是促进准备未来的研究。为此，这个核心旨在试行 人体内骨折的动物模型，自然发展出创伤后骨关节炎（PTOA）。 该模型不仅将允许对继发于PTOA的疾病进展过程的纵向研究 关节内骨折，但还将允许对新的器官治疗作用进行以后的研究 治疗范例，包括将在项目1和2中研究的生物药物干预措施。 膝盖不稳定性的猪模型也将被试验。除了这些任务外，此核心还将收集和存储 项目3和4中所有患者的血清样本，以实现未来对可能的遗传危险因素的研究 用于人类创伤后的OA。