Biomechanics and Imaging Core

生物力学和成像核心

• 批准号: 7347203
• 负责人: THOMAS DUDLEY BROWN
• 金额: $ 42.31万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-10 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7347203
• 关键词: Animal Model; Animals; Biomechanics; Cartilage; Cartilage injury; Contusions; Custom; Degenerative polyarthritis; Device Designs; Disease Progression; Elevation; Experimental Models; Family suidae; Fracture; Future; Human; Image; Intervention; Iowa; Joint Instability; Knee; Longitudinal Studies; Measurement; Modeling; Monitor; Operative Surgical Procedures; Organ; Oryctolagus cuniculus; Patients; Pharmacologic Substance; Physiologic pulse; Preparation; Process; Pulse taking; Rate; Sampling; Secondary to; Serum; Stress; Therapeutic Effect; Time; Tissue Banks; Tissues; Universities; absorption; bone; cost; genetic risk factor; tissue preparation; transmission process

A Tissue & Experimental Modeling Core will coordinate specialized tissue preparations and animal models, and will serve Projects 1, 2, and 3 of the University of Iowa CORT. All animal modeling studies will be managed by this Core, to maximize the efficiency in time, cost, and effort. This Core will provide two survival animal models: a porcine knee model of blunt impaction cartilage injury (the associated studies are proposed in Projects 1, 2, and 3), and a rabbit knee model of blunt impaction with joint instability (for Project 2). Surgical insults for these models will be applied arthroscopically, to minimize potential effects of surgical invasion itself. Impaction insults will be performed using a custom-designed device that allows delivery of a compressive force pulse of prescribed energy magnitude. The force transmission during the impaction will be monitored, allowing accurate measurement of energy absorption, the peak cartilage stress level, and the rate of cartilage stress elevation. All tissue collection from experimental animals, including cartilage and serum samples, and the processing and storing of these samples, will also be managed by this Core. Cartilage sample preparations for ex vivo studies in Projects 1, 2, and 3, namely the modeling of blunt impaction cartilage damage associated with intraarticular fractures or bone bruises, will also be provided by this Core. Another objective of this Core is to facilitate preparation for future studies. To this end, this Core aims to pilot an animal model of human intraarticular fracture that naturally develops post-traumatic osteoarthritis (PTOA). This model will allow not only longitudinal study of the disease progression processes of PTOA secondary to intraarticular fractures, but will also allow future studies of the organ-level therapeutic effects of new treatment paradigms, including bio-pharmaceutical interventions that will be studied in Projects 1 and 2. A porcine model of knee instability will also be piloted. In addition to these tasks, this Core will collect and store serum samples from all patients in Projects 3 and 4, to enable future studies of possible genetic risk factors for post-traumatic OA in humans.

组织和实验建模核心将协调专门的组织制备和动物模型；