A Cs Irradiator to Support Various Projects in Molecular Biology at UNTHSC

描述（由申请人提供）：近年来，位于沃斯堡的北德克萨斯大学健康科学中心在项目和赠款资金流入方面经历了前所未有的增长。支持资助项目的核心设施的发展有些滞后。在这个共享仪器赠款申请，它表明，NIH资助的项目在校园和其他大学在沃斯堡/阿灵顿地区可以受益显着的多功能137 Cs γ辐照器的可用性。在技术上容易引入定义明确的细胞损伤的能力将在各种项目和各种实验系统中得到利用。某些项目直接涉及研究DNA损伤反应，而其他项目则仅将这种类型的电离辐射用作工具，例如为了引入突变。例如，在个别项目中使用伽马辐照：1）诱导芽殖酵母的细胞周期检查点和转录变化。2)以允许用于免疫学研究的骨髓操作。3)在蛋白激酶C的研究中作为凋亡触发剂。4)将氧化应激引入癌症相关解毒系统的研究以及与衰老和阿尔茨海默病有关的研究中。5)作为一种方便和广泛接受的方式引入C.优雅几个二级用户也被确定，并希望访问这一新颖的和重要的资源，不仅会导致更成功的赠款申请，但也扩大了校园内的研究工作的范围。该资源的特点不是运营成本高或运营后勤困难。该仪器机械结构简单，坚固耐用，免维护。辐照时间很短，可以很容易地容纳大量的潜在用户。所有主要用户的个别项目都由NIH赠款资助，它们与人类健康的相关性很容易得到证实。一般而言，电离辐射的影响研究具有巨大的医学重要性，因为它经常被用作诊断和治疗剂。

DESCRIPTION (provided by applicant): In recent years, the University of North Texas Health Science Center in Fort Worth has experienced unprecedented growth in terms of programs and influx of grant funds. The development of core facilities supporting funded projects has somewhat lagged behind. In this shared instrument grant application, it is demonstrated that NIH funded projects on campus and at other universities in the Fort Worth/Arlington area can benefit significantly from the availability of a versatile 137Cs gamma irradiator. The capability to introduce with technical ease well-defined cellular damage is going to be exploited in a variety of projects and a variety of experimental systems. Certain projects are directly involved in studying DNA damage responses while others use this type of ionizing radiation merely as a tool, e.g. in order to introduce mutations. For instance, gamma irradiation in individual projects is used: 1) to induce cell cycle checkpoints and transcriptional changes in budding yeast. 2) to permit bone marrow manipulations for immunological studies. 3) as an apoptosis triggering agents in studies of protein kinase C. 4) to introduce oxidative stress in studies of cancer-relevant detoxification systems and in studies concerned with aging and Alzheimer's disease. 5) as a convenient and widely-accepted way to introduce mutations in C. elegans. Several secondary users have also been identified and it is hoped that access to this novel and important resource will not only lead to more successful grant applications but also to a widening of scope of research endeavors on campus. The resource is not characterized by high operating costs or difficult operational logistics. This instrument is mechanically simple, robust and maintenance-free. Irradiation times are brief and a large group of potential users can easily be accommodated. The individual projects of all primary users are funded by NIH grants, and their relevance for human health can easily be confirmed. In general, the study of effects of ionizing radiation is of tremendous medical importance because of its frequent use as a diagnostic and therapeutic agent.