NMR Investigation of Nucleotide Interactions with Cations and Proteins

核苷酸与阳离子和蛋白质相互作用的核磁共振研究

• 批准号: 7425834
• 负责人: ZHIYAN SONG
• 金额: $ 6.97万
• 依托单位: SAVANNAH STATE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7425834
• 关键词: ATP Hydrolysis; ATP phosphohydrolase; Actins; Address; Adenine; Affect; Affinity; Binding; Biological; Biological Process; Biology; Bos taurus; Bovine Serum Albumin; CCL7 gene; Cations; Cattle; Chemicals; Chemistry; Competitive Binding; Complex; Data; Diffusion; Dissociation; Extramural Activities; Funding; Guanine; Guanosine Diphosphate; Guanosine Triphosphate; Guanosine Triphosphate Phosphohydrolases; Hydrolysis; International; Investigation; Ionic Strengths; Ions; Journals; Measurement; Myosin ATPase; NMR Spectroscopy; Nucleotides; Peer Review; Pilot Projects; Play; Protein NMR Spectroscopy; Proteins; Publications; Publishing; Regulation; Relaxation; Research; Research Project Grants; Role; Sampling; Science; Score; Serum Albumin; Solutions; Structure; Students; System; Training; Ubiquitin; Universities; Variant; base; inorganic phosphate; mouse Gdi2 protein; programs; small molecule; symposium

Nucleotides such as ATP and GTP play a pivotal role in many biological activities. Nucleotide-cation interactions are essential for the stabilization, function and regulation of nucleotides. The specific and often highly selective interactions of nucleotides with the proteins are required to perform their biological functions. This proposal aims at characterizing the interactions of nucleotides with cations and proteins by NMR spectroscopy. The specific aims of the proposed research are to investigate: (1) the interactions of nucleotides with cations; (2) the mechanisms involved in the interactions of ATP/ADP with myosin and actin; and (3) the binding of ATP/GTP to bovine serum albumin (BSA) or ubiquitin. The studies involve NMR chemical shifts, T1, T2 relaxation and diffusion measurements. The NMR data will provide valuable information: a) to assess the stability of nucleotides; b) on the mechanisms of GTP/ATP hydrolysis; c) to evaluate the interplay of nucleotide-myosin/actin interactions in the ATP-driven cyclic associationdissociation of actin-myosin; and d) to verify whether BSA and ubiquitin are the candidates as ATPase or GTPase to induce ATP or GTP hydrolysis. These NMR studies have significance in biological and biomedical sciences. The successful studies conducted by the PI for his MBRS-SCORE Program Pilot- Project (2001-2005) using NMR facility at Savannah State Univeristy formed the basis for the proposed research. If funded, this SCORE research project would enable the PI not only to continue his already establshied research involving NMR studies but also train students from the MARC U*STAR and NSFHBCU- UP Programs. The results from this study will be used for presenting at national and international conferences, for publication in peer-reviewed journals and for seeking extramural funding to continue the research beyond the MBRS-SCORE Program period.

核苷酸如ATP和GTP在许多生物活动中起着关键作用。核苷酸阳离子 相互作用对于核苷酸的稳定、功能和调节是必不可少的。具体和经常 需要核苷酸与蛋白质的高度选择性相互作用来执行其生物学功能， 功能协调发展的该提案旨在通过以下方式表征核苷酸与阳离子和蛋白质的相互作用： 核磁共振波谱法。本研究的具体目的是探讨：（1） （2）ATP/ADP与肌球蛋白和肌球蛋白的相互作用机制， 肌动蛋白;和（3）ATP/GTP与牛血清白蛋白（BSA）或泛素的结合。这些研究涉及NMR 化学位移、T1、T2弛豫和扩散测量。核磁共振数据将提供有价值的 信息：a）评估核苷酸的稳定性; B）GTP/ATP水解的机制; c） 评估核苷酸-肌球蛋白/肌动蛋白相互作用在ATP驱动的循环缔合解离中的相互作用 d）验证BSA和泛素是否是ATP酶或 GTP酶诱导ATP或GTP水解。这些核磁共振研究在生物学和 生物医学科学PI为MBRS-SCORE计划试点开展的成功研究- 萨凡纳州立大学利用核磁共振设备的项目（2001-2005年）为拟议的 research.如果得到资助，这个SCORE研究项目将使PI不仅能够继续他已经 建立了涉及NMR研究的研究，但也培养了MARC U* 星星和NSFHBCU的学生- UP程序。这项研究的结果将用于在国家和国际会议上发表。 会议，在同行评审的期刊上发表，并寻求校外资金，以继续 MBRS-SCORE计划期间的研究。