Plcg1 integration of Vegf receptor signaling

提供的空间） 了解血管内皮生长因子（VEGT）相关分子的功能和调节 信号传导及其在血管发育中的作用已经变得越来越重要， 驱动该过程的机制与血管病理学所使用的机制相同。更好的 了解这一发展过程将是发展治疗和诊断的关键。 许多血管疾病，如心脏病、癌症和糖尿病。利用柔韧的脊椎动物 斑马鱼等遗传系统使得血管发育的研究既可行， 高效.在这个建议中，我们挑选出磷脂酶C γ 1（plcgl）作为信号的唯一积分器 来源于VEGF受体（Vegfr）。通过一系列的结构功能分析， plcgl SH2结构域和plcgl激活位点中的突变体，我们将确定这些中的每一个的必要性。 血管发育的组成部分，以及确定plcgl和vegfr之间的关键相互作用， 需要推动这一进程。

spaceprovided) Understanding the function and regulation of molecules involved in Vascular endothelial growth factor (Vegt) signaling and it's role in blood vessel development have become of increasing significance since the mechanisms that drive this process are the same as those used by vascular pathologies. A better understanding of this developmental process will be key in the development of therapies and diagnoses of many vascular disorders such as heart disease, cancer, and diabetes. The use of a pliable vertebrate genetic system such as the Zebrafish makes the study of blood vessel development both feasible and efficient. In this proposal we single out phospholipase C gamma 1 (plcgl) as the sole integrator of signals derived from Vegf receptors (Vegfr). Through a series of structure function analysis using mutated forms of plcgl SH2 domains and mutants in plcgl activation sites, we will determine the necessity of each of these components for blood vessel development, as well as identify key interactions between plcgl and Vegfrs that are needed to promote this process.