Analysis of Apigenin in Inhibiting Ovarian Tumorigenesis

芹菜素抑制卵巢肿瘤发生作用分析

• 批准号: 7260301
• 负责人: BingHua Jiang
• 金额: $ 7.11万
• 依托单位: WEST VIRGINIA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-13 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7260301
• 关键词: AKT Signaling Pathway; Accounting; Aerobic; Affect; Antioxidants; Apigenin; Bioflavonoid; Biological Models; Bladder; Breast; Cancer Etiology; Cancer Patient; Cause of Death; Cell Proliferation; Cells; Cessation of life; Chemoprevention; Chemopreventive Agent; Cisplatin/Paclitaxel; Colon; Development; Disease; Enzymes; Epidermal Growth Factor Receptor; Exposure to; Flavonoids; Food Supplements; Future; Gene Mutation; Genus Cola; Goals; Greater sac of peritoneum; Growth Factor; Health Food; Human; Hydrogen Peroxide; Lesion; Lung; Malignant Female Reproductive System Neoplasm; Malignant Neoplasms; Malignant neoplasm of ovary; Mediating; Molecular; Normal Cell; Operative Surgical Procedures; Organism; Ovarian; Oxidative Stress; Oxygen; PI3K/AKT; Pharmaceutical Preparations; Plants; Process; Production; Property; Prostate; Proto-Oncogene Proteins c-akt; Rate; Reactive Oxygen Species; Refractory; Resistance; Role; Signal Pathway; Signal Transduction; Signaling Molecule; Skin Neoplasms; Staging; Stomach Carcinoma; Superoxide Dismutase; Superoxides; Surface; Survival Rate; Symptoms; System; Tissues; Treatment Protocols; Vegetables; Western World; Woman; Work; angiogenesis; cancer cell; cancer prevention; cancer recurrence; cancer therapy; catalase; chemotherapy; cytokine; design; environmental agent; fruits and vegetables; glutathione peroxidase; improved; inhibitor/antagonist; malignant stomach neoplasm; melanoma; mouse model; neoplastic cell; ovarian cancer prevention; ovarian neoplasm; prevent; protective effect; tumor; tumor growth; tumorigenesis

DESCRIPTION (provided by applicant): Accumulating evidence shows that the increase of reactive oxygen species (ROS) and activation of PI3K signaling are commonly observed in several human cancers. It is known that antioxidants from fruits and vegetables have protective effects against lung, colon, breast, prostate, bladder, and stomach cancer. Our preliminary studies demonstrated that apigenin inhibits ovarian cancer cell proliferation and tumor growth. Apigenin is a nontoxic bioflavonoid compound from plants and vegetables. However, the mechanism underlying the cancer prevention effect of apigenin remains to be defined. We hypothesize that apigenin inhibits ovarian tumor growth and angiogenesis by inhibiting ROS production and PI3K/AKT signaling in the cancer cells. Specific Aim 1 is designed to identify the mechanism of apigenin in inhibiting PI3K and AKT signaling in ovarian cancer cells. We will analyze the upstream signaling molecules including ROS and EGFR that are inhibited by apigenin leading to the inhibition of PI3K and AKT in the cells. Specific Aim 2 is designed to determine whether apigenin inhibits ovarian tumorigenesis and angiogenesis, and whether PI3K and AKT are essential downstream molecules inhibited by apigenin for affecting ovarian tumor growth and angiogenesis. This work will identify new signaling molecules inhibited by apigenin, reveal mechanism of apigenin in inhibiting ovarian tumor growth and angiogenesis, and help to establish rational strategies for ovarian cancer prevention using apigenin in the future.

描述（由申请人提供）：越来越多的证据表明，活性氧（ROS）的增加和 PI3K 信号传导的激活在几种人类癌症中常见。众所周知，水果和蔬菜中的抗氧化剂对肺癌、结肠癌、乳腺癌、前列腺癌、膀胱癌和胃癌具有保护作用。我们的初步研究表明芹菜素可抑制卵巢癌细胞增殖和肿瘤生长。芹菜素是一种来自植物和蔬菜的无毒生物类黄酮化合物。然而，芹菜素预防癌症作用的机制仍有待明确。我们假设芹菜素通过抑制癌细胞中 ROS 的产生和 PI3K/AKT 信号传导来抑制卵巢肿瘤的生长和血管生成。具体目标 1 旨在确定芹菜素抑制卵巢癌细胞中 PI3K 和 AKT 信号传导的机制。我们将分析被芹菜素抑制的上游信号分子，包括ROS和EGFR，从而导致细胞中PI3K和AKT的抑制。具体目标2旨在确定芹菜素是否抑制卵巢肿瘤发生和血管生成，以及PI3K和AKT是否是芹菜素抑制影响卵巢肿瘤生长和血管生成的重要下游分子。这项工作将鉴定芹菜素抑制的新信号分子，揭示芹菜素抑制卵巢肿瘤生长和血管生成的机制，并有助于建立未来利用芹菜素预防卵巢癌的合理策略。

项目成果

Mechanism of vascular endothelial growth factor expression mediated by cisplatin in human ovarian cancer cells.

顺铂介导人卵巢癌细胞中血管内皮生长因子表达的机制。

• DOI: 10.1016/j.bbrc.2007.04.083
• 发表时间: 2007
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: Zhong,Xiao-Song;Liu,Ling-Zhi;Skinner,HeathD;Cao,Zongxian;Ding,Min;Jiang,Bing-Hua
• 通讯作者: Jiang,Bing-Hua

Acacetin inhibits VEGF expression, tumor angiogenesis and growth through AKT/HIF-1α pathway.

• DOI: 10.1016/j.bbrc.2011.08.091
• 发表时间: 2011-09-23
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Liu, Ling-Zhi;Jing, Yi;Jiang, Lisa L.;Jiang, Xiu-E;Jiang, Yue;Rojanasakul, Yongyut;Jiang, Bing-Hua
• 通讯作者: Jiang, Bing-Hua