A Resource Center for Tetrahymena Thermophila

嗜热四膜虫资源中心

基本信息

  • 批准号:
    7228229
  • 负责人:
  • 金额:
    $ 25.04万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2005
  • 资助国家:
    美国
  • 起止时间:
    2005-05-16 至 2009-04-30
  • 项目状态:
    已结题

项目摘要

This proposal seeks to establish a centralized repository for genetically distinct strains of Tetmhymena thermophila, a ciliated protozoan that has served as a key model for studies of eukaryotic cellular and molecular biology for more than 50 years. The stock center will 1) collect, annotate, and store experimentally useful cell lines; 2) establish a database that will provide essential information about these strains to the community at-large; and, 3) supply actively growing cultures to researchers around the world. This resource will provide a safe haven for the roughly 4000 unique strains developed over the past several decades, and at the same time, serve as an important adjunct to the ongoing Tetrahymena genome sequencing project. Activities spawned by this large-scale, NIH-funded sequencing effort are expected to result in the development of many new strains that will benefit greatly from the existence of a centralized storage and distribution facility. In addition to the establishment of a stock center, the proposal also intends to lay the groundwork for creation of a physical map of the Tetrahymena genome. This second goal is an extension of research currently being undertaken in the Principal Investigator's laboratory, and seeks to adapt the Cre-lox recombinase technology to the Tetrahymena system in order to create a panel of mid-sized (~ 4-5 Mb) overlapping deletions spanning the micronuclear genome. This set of specifically targeted, mid- sized deletions will augment an existing panel of large and small deletions already constructed in our laboratory by random mutagenesis, and will act as a scaffold on which genetic and molecular markers can be quickly aligned. The ability to create viable, homozygous deletions of virtually any size is only possible due to the unique dimorphic nuclear structure of Tetrahymena, and represents a powerful tool that will pave the way for functional genomic studies in this system and enhance the utility of the resource overall. Although a unicellular organism with simple growth requirements, Tetrahymena is endowed with unique genetic properties, and a structural and functional complexity comparable to that of higher metazoa. Not surprisingly, it has contributed enormously to our understanding of a wide range of biological phenomena including many with direct relevance to human health. The resource described in this proposal will ensure the continued use this organism for the benefit of society as a whole.
该提案旨在建立一个集中的储存库,用于遗传上不同的菌株

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

THEODORE G CLARK其他文献

THEODORE G CLARK的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('THEODORE G CLARK', 18)}}的其他基金

Sharing ASSETs: Expanding Science Opportunities in K-12 Classrooms
共享资产:扩大 K-12 课堂的科学机会
  • 批准号:
    8900657
  • 财政年份:
    2015
  • 资助金额:
    $ 25.04万
  • 项目类别:
Sharing ASSETs: Expanding Science Opportunities in K-12 Classrooms
共享资产:扩大 K-12 课堂的科学机会
  • 批准号:
    9229590
  • 财政年份:
    2015
  • 资助金额:
    $ 25.04万
  • 项目类别:
A Resource Center for Tetrahymena Thermophila
嗜热四膜虫资源中心
  • 批准号:
    9102372
  • 财政年份:
    2014
  • 资助金额:
    $ 25.04万
  • 项目类别:
ASSET: Advancing Secondary Science Education with Tetrahymena.
资产:利用四膜虫推进中等科学教育。
  • 批准号:
    8335522
  • 财政年份:
    2009
  • 资助金额:
    $ 25.04万
  • 项目类别:
ASSET: Advancing Secondary Science Education with Tetrahymena.
资产:利用四膜虫推进中等科学教育。
  • 批准号:
    8241019
  • 财政年份:
    2009
  • 资助金额:
    $ 25.04万
  • 项目类别:
ASSET: Advancing Secondary Science Education with Tetrahymena.
资产:利用四膜虫推进中等科学教育。
  • 批准号:
    8459924
  • 财政年份:
    2009
  • 资助金额:
    $ 25.04万
  • 项目类别:
ASSET: Advancing Secondary Science Education with Tetrahymena.
资产:利用四膜虫推进中等科学教育。
  • 批准号:
    7870353
  • 财政年份:
    2009
  • 资助金额:
    $ 25.04万
  • 项目类别:
ASSET: Advancing Secondary Science Education with Tetrahymena.
资产:利用四膜虫推进中等科学教育。
  • 批准号:
    8053303
  • 财政年份:
    2009
  • 资助金额:
    $ 25.04万
  • 项目类别:
Overexpression of membrane proteins in Tetrahymena
四膜虫膜蛋白的过度表达
  • 批准号:
    7053444
  • 财政年份:
    2006
  • 资助金额:
    $ 25.04万
  • 项目类别:
A RESOURCE CENTER FOR TETRAHYMENA THERMOPHILA
嗜热四膜虫资源中心
  • 批准号:
    7392023
  • 财政年份:
    2006
  • 资助金额:
    $ 25.04万
  • 项目类别:

相似海外基金

REU Site: Summer Undergraduate Research in Chemistry and Biochemistry at Miami University
REU 网站:迈阿密大学化学与生物化学暑期本科生研究
  • 批准号:
    2349468
  • 财政年份:
    2024
  • 资助金额:
    $ 25.04万
  • 项目类别:
    Continuing Grant
REU Site: Research Experiences for Community College Students in Chemistry and Biochemistry at Texas A&M University-Commerce
REU 网站:德克萨斯 A 社区学院化学和生物化学专业学生的研究经验
  • 批准号:
    2349522
  • 财政年份:
    2024
  • 资助金额:
    $ 25.04万
  • 项目类别:
    Standard Grant
Supporting Talented, Low-Income Undergraduate and Graduate Students in Chemistry and Biochemistry through Career Explorations, Research Experiences, and Scholarships
通过职业探索、研究经验和奖学金支持化学和生物化学领域有才华的低收入本科生和研究生
  • 批准号:
    2322722
  • 财政年份:
    2024
  • 资助金额:
    $ 25.04万
  • 项目类别:
    Standard Grant
Hydrogen and carbon dioxide biochemistry in the bacterial energy-transducing membrane.
细菌能量转换膜中的氢气和二氧化碳生物化学。
  • 批准号:
    BB/Y004302/1
  • 财政年份:
    2024
  • 资助金额:
    $ 25.04万
  • 项目类别:
    Research Grant
Conference: Active Learning Communities in Biochemistry
会议:生物化学主动学习社区
  • 批准号:
    2411535
  • 财政年份:
    2024
  • 资助金额:
    $ 25.04万
  • 项目类别:
    Standard Grant
Biochemistry of Eukaryotic Replication Fork and DNA Repair
真核复制叉的生物化学和 DNA 修复
  • 批准号:
    10550045
  • 财政年份:
    2023
  • 资助金额:
    $ 25.04万
  • 项目类别:
ORCC: The Role of Betaine Lipid Biochemistry in Coral Thermal Tolerance
ORCC:甜菜碱脂质生物化学在珊瑚耐热性中的作用
  • 批准号:
    2307516
  • 财政年份:
    2023
  • 资助金额:
    $ 25.04万
  • 项目类别:
    Continuing Grant
Supporting low-income student success in STEM through community, mentoring, and immersive research in biology and biochemistry
通过生物学和生物化学领域的社区、指导和沉浸式研究,支持低收入学生在 STEM 方面取得成功
  • 批准号:
    2221216
  • 财政年份:
    2023
  • 资助金额:
    $ 25.04万
  • 项目类别:
    Standard Grant
In vivo 2-photon imaging of retinal biochemistry before and after retinal organoid transplantation
视网膜类器官移植前后视网膜生物化学的体内2光子成像
  • 批准号:
    10643273
  • 财政年份:
    2023
  • 资助金额:
    $ 25.04万
  • 项目类别:
Biochemistry of Platelet Desialylation
血小板脱唾液酸化的生物化学
  • 批准号:
    10833844
  • 财政年份:
    2023
  • 资助金额:
    $ 25.04万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了