A Resource Center for Tetrahymena Thermophila
嗜热四膜虫资源中心
基本信息
- 批准号:7228229
- 负责人:
- 金额:$ 25.04万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2005
- 资助国家:美国
- 起止时间:2005-05-16 至 2009-04-30
- 项目状态:已结题
- 来源:
- 关键词:Anopheles gambiaeBiochemistryBiological MarkersBiological ModelsBiological PhenomenaBiologyCaenorhabditisCell AgingCell LineCell physiologyChromosome MappingChromosomesChromosomes, Human, Pair 19CiliataCollaborationsCollectionCommunitiesComplexDataDatabasesDepositionDevelopmentDirectoriesDrosophila genusEnsureEnvironmentFacility Construction Funding CategoryFreezingFundingGene ExpressionGenesGeneticGenetic ScreeningGenetic StructuresGenomeGenotypeGoalsGrowthHealedHealthHumanHypertextImmunologyInformation TechnologyLaboratoriesLinkLiquid substanceLocationMapsMethodsMicrobiologyMolecular GeneticsMolecular and Cellular BiologyMonitorMusMutagenesisNitrogenNuclear StructureNumbersOnline SystemsOrganismPersonal CommunicationPhenotypePhysical Map of the Human GenomePlayPrincipal InvestigatorPropertyPublished DirectoryRangeResearchResearch PersonnelResourcesRoleSamplingSecureSeminalServicesSiteSocietiesStructureStudy modelsSystemTechniquesTechnologyTelomeraseTetrahymenaTetrahymena thermophilaTimeUniversitiesUpdateVeterinary MedicineWheatWorkcollegecostfunctional genomicsgene functiongenetic analysisgenome databasegenome sequencinghealinginsightmemberphysical mappingrecombinaserepositoryscaffoldsizetelomeretooluser-friendlyweb interface
项目摘要
This proposal seeks to establish a centralized repository for genetically distinct strains of
Tetmhymena thermophila, a ciliated protozoan that has served as a key model for studies of eukaryotic
cellular and molecular biology for more than 50 years. The stock center will 1) collect, annotate, and store
experimentally useful cell lines; 2) establish a database that will provide essential information about these
strains to the community at-large; and, 3) supply actively growing cultures to researchers around the world.
This resource will provide a safe haven for the roughly 4000 unique strains developed over the past several
decades, and at the same time, serve as an important adjunct to the ongoing Tetrahymena genome
sequencing project. Activities spawned by this large-scale, NIH-funded sequencing effort are expected to
result in the development of many new strains that will benefit greatly from the existence of a centralized
storage and distribution facility. In addition to the establishment of a stock center, the proposal also intends
to lay the groundwork for creation of a physical map of the Tetrahymena genome. This second goal is an
extension of research currently being undertaken in the Principal Investigator's laboratory, and seeks to
adapt the Cre-lox recombinase technology to the Tetrahymena system in order to create a panel of mid-sized
(~ 4-5 Mb) overlapping deletions spanning the micronuclear genome. This set of specifically targeted, mid-
sized deletions will augment an existing panel of large and small deletions already constructed in our
laboratory by random mutagenesis, and will act as a scaffold on which genetic and molecular markers can be
quickly aligned. The ability to create viable, homozygous deletions of virtually any size is only possible due
to the unique dimorphic nuclear structure of Tetrahymena, and represents a powerful tool that will pave the
way for functional genomic studies in this system and enhance the utility of the resource overall. Although
a unicellular organism with simple growth requirements, Tetrahymena is endowed with unique genetic
properties, and a structural and functional complexity comparable to that of higher metazoa. Not
surprisingly, it has contributed enormously to our understanding of a wide range of biological phenomena
including many with direct relevance to human health. The resource described in this proposal will ensure
the continued use this organism for the benefit of society as a whole.
该提案旨在建立一个集中的储存库,用于遗传上不同的菌株
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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THEODORE G CLARK其他文献
THEODORE G CLARK的其他文献
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{{ truncateString('THEODORE G CLARK', 18)}}的其他基金
Sharing ASSETs: Expanding Science Opportunities in K-12 Classrooms
共享资产:扩大 K-12 课堂的科学机会
- 批准号:
8900657 - 财政年份:2015
- 资助金额:
$ 25.04万 - 项目类别:
Sharing ASSETs: Expanding Science Opportunities in K-12 Classrooms
共享资产:扩大 K-12 课堂的科学机会
- 批准号:
9229590 - 财政年份:2015
- 资助金额:
$ 25.04万 - 项目类别:
ASSET: Advancing Secondary Science Education with Tetrahymena.
资产:利用四膜虫推进中等科学教育。
- 批准号:
8335522 - 财政年份:2009
- 资助金额:
$ 25.04万 - 项目类别:
ASSET: Advancing Secondary Science Education with Tetrahymena.
资产:利用四膜虫推进中等科学教育。
- 批准号:
8241019 - 财政年份:2009
- 资助金额:
$ 25.04万 - 项目类别:
ASSET: Advancing Secondary Science Education with Tetrahymena.
资产:利用四膜虫推进中等科学教育。
- 批准号:
8459924 - 财政年份:2009
- 资助金额:
$ 25.04万 - 项目类别:
ASSET: Advancing Secondary Science Education with Tetrahymena.
资产:利用四膜虫推进中等科学教育。
- 批准号:
7870353 - 财政年份:2009
- 资助金额:
$ 25.04万 - 项目类别:
ASSET: Advancing Secondary Science Education with Tetrahymena.
资产:利用四膜虫推进中等科学教育。
- 批准号:
8053303 - 财政年份:2009
- 资助金额:
$ 25.04万 - 项目类别:
Overexpression of membrane proteins in Tetrahymena
四膜虫膜蛋白的过度表达
- 批准号:
7053444 - 财政年份:2006
- 资助金额:
$ 25.04万 - 项目类别:
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