National Resources for Imaging Mass Spectrometry

国家成像质谱资源

• 批准号: 7283074
• 负责人: CLAUDE P LECHENE
• 金额: $ 131.68万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7283074
• 关键词: Age; Alkali Metals; Area; Automation; Biochemistry; Biological; Biomedical Research; Biomedical Technology; Calcium; Cell surface; Cells; Cellular biology; Cesium; Characteristics; Collaborations; Communities; Condition; DNA; Data; Development; Diagnostic radiologic examination; Educational process of instructing; Educational workshop; Extracellular Domain; Fatty acid glycerol esters; Feasibility Studies; Floods; Functional disorder; Gene Transfer; Generations; Goals; Human; Image; Integral Membrane Protein; Iodine; Ions; Isotopes; Label; Laboratories; Lateral; Location; Mass Spectrum Analysis; Measurement; Measures; Metabolic Pathway; Metals; Methodology; Methods; Modality; Nucleic Acids; Penetration; Pharmaceutical Preparations; Physiology; Process; Proteins; Quantitative Microscopy; RNA; Range; Research; Research Personnel; Research Project Grants; Resolution; Resources; Sampling; Science; Secure; Series; Signal Transduction; Sodium; Spectrometry, Mass, Secondary Ion; Standardization; Standards of Weights and Measures; Stem cells; Surface; System; Techniques; Technology; Time; Toxicology; Tracer; Transplantation Immunology; United States National Institutes of Health; Virus; analytical method; base; biomedical scientist; experience; fatty acid transport; improved; instrument; ion source; mass spectrometer; metalloenzyme; method development; novel; protein degradation; sodium ion; software development; stable isotope; sugar; technology development; trafficking; transmission process

DESCRIPTION (provided by applicant): The core of this 'Resource' is a novel type of secondary ion mass spectrometer, the Multi-Isotope Imaging Mass Spectrometer (MIMS). Secondary ion mass spectrometry is based upon the sputtering of a few atomic layers from the surface of a sample. During the ejection process, some atoms and clusters are spontaneously ionized. These 'secondary ions' are a characteristic of the composition of the analyzed surface. In a secondary ion mass spectrometer, the secondary ions are separated according to their mass, and a quantitative image of the distribution of the selected mass is formed. MIMS provides high mass separation (M/deltaM > 10,000), high spatial resolution (< 50 nm) and has the unique capability of simultaneously recording several atomic mass images. Of the utmost importance, MINIS makes it possible for the first time (and at the intracellular level) to simultaneously image the distribution and measure the accumulation of molecules labeled with any isotopes, in particular with stable isotopes, for example with 15N. Thus, MIMS allows one to study the localization, the accumulation and the turnover of proteins, fats, sugars and foreign molecules in cellular microdomains. The Resource collaborates with researchers to use the unique advantages of MIMS to bring original information in cell biology, physiology, physiopathology, biochemistry, immunology, transplantation, toxicology, stem cell and gene transfer research. MINIS offers a powerful and quantitative new method for studying intra and transcellular metabolic pathways, signal transduction, RNA and DNA expression and distribution, fatty acid transport, donor-receiver cellular trafficking, stem cell nesting and localization of drugs. Finally, the use of stable isotopes offers a world of labeling possibilities that were impossible with auto-radiography. It will revive and expand the use of tracers in humans. Instrumental development (brighter cesium primary ion source, iodine negative primary ion source, bipolar Cs+/Isource, preanalysis covering of the sample surface with cesium) will increase the resolution to 20nm, will allow sensitive measurements of calcium, alkali metals (Na, K), metals (metalloenzymes) and will allow analysis of the very first atomic layers at the cell surface. Study of secondary ion formation, development of automation and powerful data reduction software, development of standards, and development of methods for long term labeling of cells will increase the breadth of the Resource and will benefit the community of users. A workshop has been and will be organized every year. A Resource website displays a wealth of information to the budding community of users. A secure server allows exchanges of large reams of data between the Resource and users. A webserver for the community of users is being built and will be maintained by the Resource.

描述(申请人提供)：本资源的核心是一种新型的二次离子质谱仪，多同位素成像质谱仪(MIMS)。二次离子质谱仪的基础是从样品表面溅射出几层原子层。在抛射过程中，一些原子和团簇被自发电离。这些“二次离子”是被分析表面的组成特征。在二次离子质谱仪中，二次离子根据它们的质量被分离，并形成所选质量分布的定量图像。 MIMS提供高质量分离(M/DeltaM&GT；10,000)、高空间分辨率(&lt；50 nm)，并具有同时记录多个原子质量图像的独特能力。最重要的是，MINIS使首次(在细胞内)能够同时成像和测量标记了任何同位素的分子的分布和累积，特别是标记了稳定同位素的分子，例如15N。因此，MIMS使人们能够研究蛋白质、脂肪、糖和外来分子在细胞微域中的定位、积累和周转。该资源与研究人员合作，利用MIMS的独特优势，带来细胞生物学、生理学、生理学、生化、免疫学、移植、毒理学、干细胞和基因转移研究的原始信息。Minis为研究细胞内和跨细胞代谢途径、信号转导、RNA和DNA的表达和分布、脂肪酸转运、供体-受体细胞转运、干细胞嵌套和药物定位提供了一种有效和定量的新方法。最后，稳定同位素的使用提供了标记的可能性，而这是自动放射照相无法实现的。它将复兴并扩大示踪剂在人类身上的使用。仪器开发(更明亮的铯初级离子源、碘负初级离子源、双极Cs+/Isource、用铯覆盖样品表面的分析前)将把分辨率提高到20 nm，将允许对钙、碱金属(Na、K)、金属(金属酶)进行灵敏测量，并将能够分析细胞表面的第一层原子层。研究二次离子形成，开发自动化和强大的数据还原软件，制定标准，开发细胞长期标记方法，将增加资源的广度，并将造福于用户社区。已经并将每年举办一次讲习班。资源网站向初露头角的用户社区展示了丰富的信息。安全的服务器允许资源和用户之间交换大量数据。正在为用户社区建立一个网络服务器，并将由该资源进行维护。