VIPER RESOURCE CENTER AT TEXAS A&M UNIVERSITY-KINGSVILLE

德克萨斯州 VIPER 资源中心

• 批准号: 7217247
• 负责人: John Carlos Perez
• 金额: $ 46.29万
• 依托单位: TEXAS A&M UNIVERSITY-KINGSVILLE
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-15 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7217247
• 关键词: Adhesions; Agkistrodon; Alleles; Alternative Splicing; Appendix; Biological Assay; Biomedical Research; Breeding; Cause of Death; Cell physiology; Cells; Characteristics; Coagulation Process; Complex Mixtures; Crotalus; Data; Databases; Development; Disease; Disintegrins; Dose; Enzymes; Facility Construction Funding Category; Future; Genetic Variation; Geographic Locations; Goals; High Pressure Liquid Chromatography; Housing; Human; Inhibitory Concentration 50; Internet; Ischemia; Lead; Lethal Dose 50; Liquid Chromatography; Literature; Malignant Neoplasms; Medical; Medicine; Metalloproteases; Mission; Myocardial Infarction; Numbers; Organ; Paper; Performance; Pharmaceutical Preparations; Platelet Aggregation Inhibition; Population; Procedures; Process; Property; Proteins; Protocols documentation; Publishing; Rate; Research; Research Personnel; Research Training; Resources; Sampling; Skin; Snake Venoms; Snakes; Source; Standardization; Stroke; Structure-Activity Relationship; System; Texas; Therapeutic; Time; Tissues; Titrations; Toxin; United States; Universities; Variant; Vendor; Venoms; base; biomedical resource; database design; day; enzyme activity; insight; polyclonal antibody; programs; success

DESCRIPTION (provided by applicant): New drugs are being developed each year for cancer, heart attacks, and strokes and some have been derived from snake venoms. Snake venoms are complex mixtures of molecules that can have therapeutic value in the treatment of clot-induced ischemia, cancer, and other human disorders. Snake venoms are rich, stable sources of disintegrins, metalloproteases, and fibrinolytic enzymes. The literature contains many references on how venom and/or venom components are being used in medicine. There are 44 subspecies of poisonous snakes within the U.S.. Despite the vast species diversity, 90% of the papers published have been with venom from a limited number of the more common species (Crotalus (C.) atrox, C. adamanteus, C. viridis viridis, Agkistrodon (A.) contortrix contortrix, A. contortrix laticinctus, and A. piscivorus piscivorus). Many of the snake venoms are not available for research. Therefore, venom composition and potential usefulness in medicine has not been explored. There is a need to develop a Center capable of providing quality venoms, venom fractions, tissues, organs, skins, and monoclonal and polyclonal antibodies for biomedical research. For over 28 years, TAMUK has maintained a strong venom research program and has developed a serpentarium that presently houses over 350 snakes composed of 14 species and 24 subspecies. Two years ago, the Texas A&M University System Board of Regents approved the development of a Natural Toxins Research Center (NTRC) at TAMUK. The mission of the NTRC is to provide global research, training, and resources that will lead to the discovery of medically important toxins found in venoms. The objectives of this proposal are to: 1) provide reliable sources of venoms and other snake products that are not available from other vendors; 2) breed poisonous snakes in captivity that are endangered or difficult to acquire; 3) characterize medically important venoms by electrophoretic titration (ET), high performance liquid chromatography (HPLC), enzyme activities, and cell-based assays; 4) develop a research program that provides insights into structure/function relationships of venom proteins, and 5) develop a database that will be useful to other investigators. The database will allow the user to view the snakes, their geographical distribution and venom characteristics.

描述（由申请人提供）： 每年都有新的药物被开发出来用于治疗癌症、心脏病和中风，有些药物是从蛇毒中提取的。 蛇毒是分子的复杂混合物，在治疗血栓引起的缺血、癌症和其他人类疾病方面具有治疗价值。 蛇毒是解整合素、金属蛋白酶和纤维蛋白溶解酶的丰富、稳定的来源。 该文献包含许多关于毒液和/或毒液组分如何用于医学的参考文献。 美国境内有44种毒蛇亚种。 尽管物种多样性巨大，但90%的论文发表的毒液来自数量有限的更常见的物种（响尾蛇（C.）atrox，C. adamanteus，C. viridis viridis、蝮蛇Agkistrodon（A.）contora-contoraeA.和A.食鱼的）。 许多蛇毒无法用于研究。 因此，毒液的组成和在医学上的潜在用途尚未被探索。 有必要建立一个中心，能够提供高质量的毒液，毒液组分，组织，器官，皮肤，单克隆和多克隆抗体的生物医学研究。 在过去的28年里，TAMUK一直保持着强大的毒液研究计划，并开发了一个蛇馆，目前拥有超过350条蛇，包括14个物种和24个亚种。 两年前，德克萨斯A&M大学系统董事会批准在TAMUK建立天然毒素研究中心（NTRC）。 NTRC的使命是提供全球性的研究、培训和资源，以发现毒液中的重要医学毒素。本提案的目的是：1）提供其他供应商无法提供的毒液和其他蛇类产品的可靠来源; 2）圈养濒危或难以获得的毒蛇; 3）通过电泳滴定（ET）、高效液相色谱（HPLC）、酶活性和基于细胞的测定来表征医学上重要的毒液; 4）开发一个研究计划，提供对毒液蛋白质结构/功能关系的见解，以及5）开发一个对其他研究人员有用的数据库。 该数据库将允许用户查看蛇、它们的地理分布和毒液特征。