Protein Chemistry Laboratory Core

蛋白质化学实验室核心

• 批准号: 7315557
• 负责人: JOHN D LAMBRIS
• 金额: $ 23.23万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7315557
• 关键词: Animals; Antibodies; Architecture; Biochemical; Bioinformatics; Biological Assay; Blood; C5a anaphylatoxin receptor; Cell Fractionation; Cell Proliferation; Cells; Collection; Complement; Complement 3a; Complement Receptor; Coupling; Disease model; Electrophoresis; Experimental Models; Genes; Genetic; High Pressure Liquid Chromatography; Image; Injury; Laboratories; Liquid Chromatography; MALDI-TOF Mass Spectrometry; Mass Spectrum Analysis; Modeling; Molecular; Monitor; Mus; Organelles; Pattern; Peptides; Phosphopeptides; Phosphorylation; Plasma; Preparation; Procedures; Protein Analysis; Protein Chemistry; Proteins; Proteome; Proteomics; Purpose; Reagent; Regulation; Resources; Serum; Serum Proteins; Services; Signal Transduction; Site; Specimen; Spectrometry; Staging; Subcellular Fractions; Surface Plasmon Resonance; System; Techniques; Technology; Time; Tissues; base; cytokine; design; direct application; fast protein liquid chromatography; in vivo; interest; ionization; mass spectrometer; member; neutralizing antibody; polyclonal antibody; programs; protein expression; protein protein interaction; receptor; reconstitution; research study; synthetic peptide; tool; two-dimensional

The Protein Chemistry Laboratory Core will serve as the major protein preparation and analysis resource for the Program Project. The laboratory will be responsible for protein preparation, characterization and identification which will include hypothesis and project driven proteomics. The Protein Chemistry Laboratory is equipped with three automated peptide synthesizers, an automated Edman sequencer, various HPLC and FPLC systems encompassing both micro and macro scale. Most importantly the laboratory has two "proteomics-appropriate" mass spectrometry systems equipped with ionization modes that will enable sub-picomole analysis of proteins and peptides with bio-informatics support. All protein and peptide products will be subjected to stringent characterization by mass spectrometric/ proteomic analysis. Surface Plasmon resonance (Biacore) analysis is also available that is adaptable to coupling with the on-site mass spectrometers. The primary services provided will be the provision of protein-related tools and reagents such as purified murine complement system proteins, monoclonal and polyclonal antibodies, synthetic peptides, and peptide-like synthetic antagonists that interrupt various and specific complement system protein-protein interactions. Hypothesis driven proteomics will also be applied to delineate molecular and biochemical mechanisms underlying the various complement regulated disease models. All members of the program project will be served by the PCLC.

蛋白质化学实验室核心将作为主要的蛋白质制备和分析 计划项目的资源。实验室将负责蛋白质的制备， 表征和鉴定，包括假说和项目驱动的蛋白质组学。这个 蛋白质化学实验室配备了三台自动多肽合成器，一台自动化的 埃德曼测序仪，包括微观和宏观尺度的各种高效液相色谱仪和FPLC系统。 最重要的是，该实验室有两个“适合蛋白质组学”的质谱学系统 配备了电离模式，使蛋白质和多肽的亚皮摩尔分析成为可能 生物信息学支持。所有蛋白质和多肽产品都将受到严格的 通过质谱学/蛋白质组学分析进行表征。表面等离子激元共振(Biacore) 还提供了适合与现场质谱计耦合的分析。 提供的主要服务将是提供与蛋白质有关的工具和试剂，如 纯化的小鼠补体系统蛋白，单抗和多克隆抗体，人工合成 中断各种和特定补体的多肽和多肽类合成拮抗剂 系统蛋白质-蛋白质相互作用。假说驱动的蛋白质组学也将被应用于描绘 补体调节性疾病的分子和生化机制 模特们。该计划项目的所有成员将由PCLC提供服务。