Protein Chemistry Laboratory Core

蛋白质化学实验室核心将作为主要的蛋白质制备和分析 项目的资源。实验室将负责蛋白质制备， 表征和鉴定，包括假设和项目驱动的蛋白质组学。的 蛋白质化学实验室配备了三台自动肽合成仪，一台自动 Edman测序仪，各种HPLC和FPLC系统，包括微观和宏观尺度。 最重要的是，该实验室有两个“蛋白质组学适用”的质谱系统 配备电离模式，可对蛋白质和肽进行亚皮摩尔分析， 生物信息学支持。所有蛋白质和肽产品将进行严格的 通过质谱/蛋白质组学分析表征。表面等离子体共振（Biacore） 也可进行适于与现场质谱仪耦合的分析。 提供的主要服务将是提供与蛋白质有关的工具和试剂，例如 纯化的鼠补体系统蛋白，单克隆和多克隆抗体，合成的 肽和肽样合成拮抗剂， 系统蛋白质相互作用。假设驱动的蛋白质组学也将被应用于描绘 各种补体调节疾病的分子和生化机制 模型该计划项目的所有成员将由PCLC提供服务。

The Protein Chemistry Laboratory Core will serve as the major protein preparation and analysis resource for the Program Project. The laboratory will be responsible for protein preparation, characterization and identification which will include hypothesis and project driven proteomics. The Protein Chemistry Laboratory is equipped with three automated peptide synthesizers, an automated Edman sequencer, various HPLC and FPLC systems encompassing both micro and macro scale. Most importantly the laboratory has two "proteomics-appropriate" mass spectrometry systems equipped with ionization modes that will enable sub-picomole analysis of proteins and peptides with bio-informatics support. All protein and peptide products will be subjected to stringent characterization by mass spectrometric/ proteomic analysis. Surface Plasmon resonance (Biacore) analysis is also available that is adaptable to coupling with the on-site mass spectrometers. The primary services provided will be the provision of protein-related tools and reagents such as purified murine complement system proteins, monoclonal and polyclonal antibodies, synthetic peptides, and peptide-like synthetic antagonists that interrupt various and specific complement system protein-protein interactions. Hypothesis driven proteomics will also be applied to delineate molecular and biochemical mechanisms underlying the various complement regulated disease models. All members of the program project will be served by the PCLC.