Compositional Analysis of the Arabidopsis 26S Proteasome

拟南芥 26S 蛋白酶体的组成分析

• 批准号: 7364569
• 负责人: Adam J Book
• 金额: $ 2.64万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7364569
• 关键词: 26S proteasome; Agriculture; Amino Acids; Arabidopsis; Arabidopsis Proteins; Binding; Complex; Defect; Depth; Development; Disease; Endopeptidases; Eukaryota; Eukaryotic Cell; Excision; Genes; Goals; Hormonal; Individual; Knowledge; Lead; Localized; Maintenance; Medicine; Methods; Modeling; Molecular; Mouse-ear Cress; Organism; Pathway interactions; Peptide Hydrolases; Peptides; Physiological; Physiology; Plants; Protein Isoforms; Proteins; Research; Role; Signal Transduction; Substrate Specificity; System; Testing; Tissues; Ubiquitin; Variant; Work; Yeasts; feeding; genetic regulatory protein; in vivo; multicatalytic endopeptidase complex; protein degradation; response

spaceprovided) The goal of this research is to reveal the molecular composition and functions of the 26S proteasome in a multicellular eukaryote, using Arabidopsis thaliana as the model. Organisms rely on protein degradation for a variety of essential functions, including the maintenance of free amino acid pools, the removal of damaged proteins, and the control of regulatory protein abundance. The major machine for this breakdown is the 26S proteasome, an ATP-dependent 2-MDa proteolytic complex. The specific aim of this project is to define the composition of the native 26S proteasome from Arabidopsis, reveal the functions of various subunits, and determine whether the plant preferentially assembles specific subtypes of the complex through selective pairing of various isoforms. A more complete characterization of the 26S proteasome will lead to a deeper understanding of how proteins are selectively degraded which may potentially identify new ways to control protein breakdown in vivo. Given the importance of Ub and the 26S proteasome to almost all facets of eukaryotic physiology and development, this work should have practical benefits to both medicine and agriculture, including various disease caused by defects in the Ubiquitin/26S proteasome system.

提供的空间） 本研究的目的是揭示26 S蛋白酶体的分子组成和功能， 以拟南芥为模型的多细胞真核生物。生物体依靠蛋白质降解 多种基本功能，包括维持游离氨基酸库，清除受损的 蛋白质，以及调节蛋白质丰度的控制。造成这种故障的主要机器是26 S 蛋白酶体，一种ATP依赖的2-MDa蛋白水解复合物。该项目的具体目标是确定 拟南芥天然26 S蛋白酶体的组成，揭示了各种亚基的功能， 确定所述植物是否通过选择性地组装所述复合物的特定亚型， 各种异构体的配对。26 S蛋白酶体的更完整表征将导致更深入的研究。 了解蛋白质是如何选择性降解的，这可能会发现新的控制方法。 体内蛋白质分解。考虑到Ub和26 S蛋白酶体对几乎所有方面的重要性， 真核生物的生理学和发育，这项工作应该有实际的好处， 农业，包括由泛素/26 S蛋白酶体系统缺陷引起的各种疾病。