Development of Passive Microrheology as a tool to study cell substrate interaction

开发被动微流变学作为研究细胞基质相互作用的工具

• 批准号: EP/E015395/1
• 负责人: Athene Donald DBE FRS
• 金额: $ 19.54万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FE015395%2F1
• 关键词: Development; Passive; Microrheology; tool; study

We propose to use new micromechanical techniques of microrheology to assess to what extent they are able to add new physical non-invasive methodologies for studying cell interaction with substrates given that so much of cell biology is dependent on destructive analysis. To assist in this we are going to work with two cells, keratinocytes and fibroblasts, looking at their interaction with a small range of defined substrates under physiological and sub-physiological calcium conditions. The interactions of these cells with these substrates under these conditions is already well known and well documented in the MacNeil laboratory. Dr Sally McArthur will be providing the well characterised substrates for this project. Accordingly we will use a range of well established cell interactions with substrates to examine to what extent microrheology can usefully follow the behaviour of cells as they interact with substrates and hopefully be used to predict the organisation of the cytoskeleton without having to resort to destructive testing.

我们建议使用新的微流变学的微机械技术，以评估在何种程度上，他们能够添加新的物理非侵入性的方法来研究细胞与基板的相互作用，这么多的细胞生物学是依赖于破坏性分析。为了帮助这一点，我们将与两种细胞，角质形成细胞和成纤维细胞，在生理和亚生理钙条件下，观察它们与小范围的限定底物的相互作用。在这些条件下，这些细胞与这些底物的相互作用已经是众所周知的，并且在MacNeil实验室中得到了很好的记录。Sally麦克阿瑟博士将为该项目提供良好表征的基质。因此，我们将使用一系列良好建立的细胞与底物的相互作用，以检查在何种程度上微流变学可以有效地遵循细胞的行为，因为它们与底物相互作用，并希望被用来预测组织的细胞骨架，而不必诉诸破坏性测试。

项目成果

Gels formed from amino-acid derivatives, their novel rheology as probed by bulk and particle tracking rheological methods

• DOI: 10.1016/j.jnnfm.2014.09.008
• 发表时间: 2015-08-01
• 期刊: JOURNAL OF NON-NEWTONIAN FLUID MECHANICS
• 影响因子: 3.1
• 作者: Frith, W. J.;Donald, A. M.;Aufderhorst-Roberts, A.
• 通讯作者: Aufderhorst-Roberts, A.

A micro-incubator for cell and tissue imaging.

用于细胞和组织成像的微型培养箱。

• DOI: 10.2144/000113245
• 发表时间: 2010
• 期刊: BioTechniques
• 影响因子: 2.7
• 作者: Picard C