BIOLOGICAL TESTING CORE

备用生物试验核心设施全面投入运作 目前。我们已经获得了必要的过滤设备，蝌蚪测试设备， 膜制备设备。研究单位的共用设施将免费提供使用， 包括离心机（低速、中速和高速）、冷藏室、伽马和闪烁计数器 和冰柜。 2.F.生物试验核心工厂引用的文献。 [1]Covey，DF，Nathan，D，Kalkbrenner，M，Nilsson，KR，Hu，Y，Zorumski，CF和Evers，AS， 孕烯醇酮诱导的γ-氨基丁酸（A）受体调节的对映体选择性和 麻醉药理学与实验治疗学杂志。293：1009-1016，2000. [2]Franks，NP和Lieb，WR，挥发性全身麻醉剂在分子和细胞中的选择性作用 程度.英国麻醉学杂志。71：65-76，1993. [3]Gee，KW和Lan，NC，大鼠额叶皮层中的γ-氨基丁酸A受体复合物， 脊髓对类固醇调节表现出不同反应。分子药理学。40：995- 999，1991。 [4]哈尔西，MJ，Wardley-Smith，B和Wood，S，阿法沙龙/阿法多龙的压力逆转和 蝌蚪中的美索己酮：全身麻醉不同分子位点的证据。英国 药理学杂志。89：299-305，1986. [5]Mauriska，MD，Harrison，NL，Schwartz，RD，Barker，JL和Paul，SM，类固醇激素代谢物 是GABA受体的巴比妥类调节剂。科学232：1004-1007，1986. [6]Paradiso，K，Sabey，K，Evers，AS，Zorumski，CF，Covey，DF和施泰因巴赫，JH，类固醇抑制 大鼠神经元烟碱α 4 β 2受体在HEK 293细胞中表达。分子药理学。 58：341- 351，2000。 [7]Srinivasan，S，Sapp，DW，Tobin，AJ和Olsen，RW，GABA受体结合的双相调节 表明了功能相关性。神经化学研究。24：1363-1372，1999. [8]Tonner，PH，Poppers，DM和米勒，KW，异丙酚的全身麻醉效力及其对 取决于静水压力。麻醉学。77：926-931，1992. [9]Turner，DM，Ransom，RW，Yang，JS和Olsen，RW，类固醇麻醉剂和天然类似物 调节γ-氨基丁酸受体复合物，

for use. The Biological Testing Core Facility is fully operational at present. We have acquired the necessary filtration apparatus, testing equipment for tadpoles and equipment for membrane preparations. The Research Unit shared facilities will be freely available for use, including centrifuges (low-, intermediate- and high-speed), cold room, gamma and scintillation counters and freezers. 2.F. LITERATURE CITED FOR THE BIOLOGICAL TESTING CORE FACILITY. [1] Covey, DF, Nathan, D, Kalkbrenner, M, Nilsson, KR, Hu, Y, Zorumski, CF and Evers, AS, Enantioselectivity of pregnanolone-induced gamma-aminobutyric acid(A) receptor modulation and anesthesia. Journal of Pharmacology and Experimental Therapeutics. 293:1009-1016, 2000. [2] Franks, NP and Lieb, WR, Selective actions of volatile general anaesthetics at molecular andcellular levels. British Journal of Anaesthesia. 71:65-76, 1993. [3] Gee, KW and Lan, NC, Gamma-aminobutyric acidA receptor complexes in rat frontal cortex and spinal cord show differential responses to steroid modulation. Molecular Pharmacology. 40:995- 999, 1991. [4] Halsey, MJ, Wardley-Smith, B and Wood, S, Pressure reversal of alphaxalone/alphadolone and methohexitone in tadpoles: evidence for different molecular sites for general anaesthesia. British Journal of Pharmacology. 89:299-305, 1986. [5] Majewska, MD, Harrison, NL, Schwartz, RD, Barker, JL and Paul, SM, Steroid hormone metabolites are barbiturate-like modulators of the GABA receptor. Science. 232:1004-1007, 1986. [6] Paradiso, K, Sabey, K, Evers, AS, Zorumski, CF, Covey, DF and Steinbach, JH, Steroid inhibition of rat neuronal nicotinic a4 B2 receptors expressed in HEK 293 cells. Molecular Pharmacology. 58:341-351,2000. [7] Srinivasan, S, Sapp, DW, Tobin, AJ and Olsen, RW, Biphasic modulation of GABA receptor binding by steroids suggests functional correlates. Neurochemical Research. 24:1363-1372, 1999. [8] Tonner, PH, Poppers, DM and Miller, KW, The general anesthetic potency of propofol and its dependence on hydrostatic pressure. Anesthesiology. 77:926-931, 1992. [9] Turner, DM, Ransom, RW, Yang, JS and Olsen, RW, Steroid anesthetics and naturally occurring analogs modulate the gamma-aminobutyric acid receptor complex at a