iCys Laser Scanning Cytometer for UC Davis Core

适用于 UC Davis Core 的 iCys 激光扫描细胞仪

• 批准号: 7390044
• 负责人: Janine M LaSalle
• 金额: $ 50万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-18 至 2009-04-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7390044
• 关键词: Area; Ataxia; Autistic Disorder; Biology; Biomedical Research; Blindness; Childhood; Computer Systems; Contract Services; Core Facility; Dementia; Diabetes Mellitus; Disease; Elements; Equipment; Flow Cytometry; Fluorescent in Situ Hybridization; Funding; Generations; Goals; Grant; Health system; Image; Image Analysis; Inclusion Bodies; Lasers; Life; Malignant Neoplasms; Mental Retardation; Microscope; Microscopy; Modeling; Neurons; Nuclear; Nuclear Protein; Nuclear Proteins; Optics; Organelles; Placement; Process; Protein Dynamics; Publications; Research; Research Personnel; Scanning; Slide; Spottings; System; Technology; United States National Institutes of Health; Update; age related; cellular imaging; cost effective; high throughput screening; medical schools; programs

DESCRIPTION (provided by applicant): The CompuCyte iCys Laser Scanning Cytometer is a versatile research imaging microscope that combines elements of flow cytometry, automated fluorescent microscopy, and image analysis. A first generation Compucyte Laser Scanning Cytometer (LSC) was purchased by the UC Davis School of Medicine in 1997 for shared use within the Optical Biology Core facility. In the last 10 years, the LSC has been actively used at UC Davis, resulting in more than 15 publications and 12 new grants using the technology. Currently, 9 funded NIH grants from 6 investigators are dependent on an operational LSC. Starting in mid-2007, the older model LSC will no longer be supported by CompuCyte, so the purchase of a new iCys LSC is essential to the completion of these funded projects. In addition, the new iCys LSC not only has an updated software and computer system, but is in an inverted microscope format, allowing new applications to include live cell imaging, 96-well plate high throughput assays, and multi-slide processing. Institutional support is provided for an extended service contract and a confocal module on the iCys LSC to allow the high-throughput quantitative analyses of small intra-nuclear spots in fluorescence in situ hybridization, sub-nuclear protein dynamics, intra-cellular inclusions, and organelle studies in primary neurons. There is no equivalent equipment on the UC Davis campus or surrounding area that would serve the quantitative imaging needs of these NIH-funded programs. The iCys LSC with confocal module will strongly impact inter-disciplinary biomedical research on the common pediatric disorders of autism and mental retardation, as well as the common age-related disorders of dementia, ataxia, blindness, cancer, and diabetes. These are common goals of the UC Davis Health System and the NIH. The placement of a shared iCys LSC imaging system in a core facility with a strong track record of support is a cost effective way of supporting NIH funded researchers at UC Davis.

描述（由申请人提供）：CompuCyte iCys激光扫描细胞仪是一种多功能的研究成像显微镜，结合了流式细胞术、自动荧光显微镜和图像分析的元素。加州大学戴维斯分校医学院于1997年购买了第一代Compucyte激光扫描细胞仪（LSC），用于光学生物学核心设施的共享使用。在过去的10年里，加州大学戴维斯分校一直在积极使用LSC，使用该技术发表了超过15篇论文，并获得了12项新的资助。目前，来自6名研究人员的9笔NIH资助资金依赖于一个可操作的LSC。从2007年年中开始，CompuCyte将不再支持旧型号的LSC，因此购买新的iCys LSC对于完成这些资助项目至关重要。此外，新的iCys LSC不仅具有更新的软件和计算机系统，而且具有倒置显微镜格式，允许新的应用包括活细胞成像，96孔板高通量测定和多载玻片处理。为iCys LSC的扩展服务合同和共聚焦模块提供机构支持，以允许高通量定量分析荧光原位杂交的小核内斑点，亚核蛋白动力学，细胞内包涵体和初级神经元的细胞器研究。加州大学戴维斯分校校园或周边地区没有相应的设备可以满足这些美国国立卫生研究院资助项目的定量成像需求。具有共聚焦模块的iCys LSC将对自闭症和智力迟钝等常见儿科疾病以及痴呆、共济失调、失明、癌症和糖尿病等常见年龄相关疾病的跨学科生物医学研究产生重大影响。这些都是加州大学戴维斯分校卫生系统和美国国立卫生研究院的共同目标。将共享iCys LSC成像系统放置在具有强大支持记录的核心设施中，是支持美国国立卫生研究院资助的加州大学戴维斯分校研究人员的一种经济有效的方式。