iCys Laser Scanning Cytometer for UC Davis Core

描述(申请人提供)：CompuCyte ICYS激光扫描细胞仪是一种多功能研究成像显微镜，它结合了流式细胞术、自动荧光显微镜和图像分析的元素。加州大学戴维斯分校医学院于1997年购买了第一代Compucyte激光扫描细胞仪(LSC)，供光学生物学核心设施共享使用。在过去的10年里，加州大学戴维斯分校积极使用LSC，使用该技术出版了15本以上的出版物和12笔新的赠款。目前，来自6名调查人员的9笔由NIH资助的赠款依赖于一个可运作的LSC。从2007年年中开始，旧型号的LSC将不再得到CompuCyte的支持，因此购买新的ICYS LSC对于完成这些资助的项目是必不可少的。此外，新的ICYS LSC不仅拥有最新的软件和计算机系统，而且是倒置显微镜格式，允许新的应用包括活细胞成像、96孔板高通量分析和多载玻片处理。为ICYS LSC的延长服务合同和共聚焦模块提供了机构支持，以便高通量地对荧光原位杂交、亚核蛋白质动力学、细胞内包涵体和初级神经元细胞器研究中的核内小斑点进行高通量定量分析。加州大学戴维斯分校或周边地区没有同等的设备来满足这些NIH资助项目的定量成像需求。具有共焦模块的ICYS LSC将强烈影响对自闭症和智力低下等常见的儿科疾病以及痴呆症、共济失调、失明、癌症和糖尿病等常见的与年龄相关的疾病的跨学科生物医学研究。这些都是加州大学戴维斯分校健康系统和美国国立卫生研究院的共同目标。将共享的ICYS LSC成像系统放置在具有良好支持记录的核心设施中，是支持加州大学戴维斯分校NIH资助的研究人员的一种经济高效的方式。

DESCRIPTION (provided by applicant): The CompuCyte iCys Laser Scanning Cytometer is a versatile research imaging microscope that combines elements of flow cytometry, automated fluorescent microscopy, and image analysis. A first generation Compucyte Laser Scanning Cytometer (LSC) was purchased by the UC Davis School of Medicine in 1997 for shared use within the Optical Biology Core facility. In the last 10 years, the LSC has been actively used at UC Davis, resulting in more than 15 publications and 12 new grants using the technology. Currently, 9 funded NIH grants from 6 investigators are dependent on an operational LSC. Starting in mid-2007, the older model LSC will no longer be supported by CompuCyte, so the purchase of a new iCys LSC is essential to the completion of these funded projects. In addition, the new iCys LSC not only has an updated software and computer system, but is in an inverted microscope format, allowing new applications to include live cell imaging, 96-well plate high throughput assays, and multi-slide processing. Institutional support is provided for an extended service contract and a confocal module on the iCys LSC to allow the high-throughput quantitative analyses of small intra-nuclear spots in fluorescence in situ hybridization, sub-nuclear protein dynamics, intra-cellular inclusions, and organelle studies in primary neurons. There is no equivalent equipment on the UC Davis campus or surrounding area that would serve the quantitative imaging needs of these NIH-funded programs. The iCys LSC with confocal module will strongly impact inter-disciplinary biomedical research on the common pediatric disorders of autism and mental retardation, as well as the common age-related disorders of dementia, ataxia, blindness, cancer, and diabetes. These are common goals of the UC Davis Health System and the NIH. The placement of a shared iCys LSC imaging system in a core facility with a strong track record of support is a cost effective way of supporting NIH funded researchers at UC Davis.